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Series GSE53918 Query DataSets for GSE53918
Status Public on Jun 30, 2014
Title Differentiation of human limbal-derived induced pluripotent stem cells into limbal-like epithelium
Organism Homo sapiens
Experiment type Methylation profiling by array
Summary Limbal epithelial stem cell (LESC) deficiency represents a significant clinical problem especially in bilateral cases. Induced pluripotent stem cells (iPSC) may be a promising source of LESC, allowing standardized and continual propagation and banking. The objective of this study was to generate iPSC from human limbal epithelial cultures and differentiate them back into limbal epithelial cells using substrata mimicking the natural LESC niche. Using Yamanaka’s episomal vectors limbal-derived iPSC were reprogrammed from LESC cultured from donor corneoscleral rims and from human skin fibroblasts. A clone from limbal-derived iPSC expressed stemness markers, had a diploid karyotype, and produced teratomas in nude mice representing three germ layers. Compared to parental LESC, this clone had fewer specific gene methylation changes revealed using the Illumina Infinium Methylation 450k Beadchips than compared to skin fibroblasts. The expression of putative LESC markers was examined by quantitative RT-PCR and immunostaining in limbal-derived and fibroblast-derived iPSC cultured on denuded human amniotic membrane or denuded cornea. Limbal-derived iPSC had markedly stronger expression of PAX6, ABCG2, Np63, keratins 14, 15, 17, and N-cadherin than fibroblast-derived iPSC. On denuded corneas, limbal-derived iPSC showed the expression of differentiated corneal keratins 3 and 12. The data suggest that iPSC differentiation to a desired lineage may be facilitated by their generation from the same tissue. This may be related to preservation of parental tissue epigenetic methylation signatures in iPSC and use of biological substrata similar to the natural niche of parental cells. The data pave the way for generating transplantable LESC from limbal-derived iPSC.
Overall design Bisulphite converted DNA from the 12 samples were hybridised to the Illumina Infinium 450k Human Methylation Beadchip
Contributor(s) Sareen D, Saghizadeh M, Tang J, Funari VA, Punj V, Svendsen CN, Ljubimov AV
Citation(s) 25069777
Submission date Jan 08, 2014
Last update date Mar 22, 2019
Contact name Jie Tang
Organization name Cedars Sinai Medical Center
Street address 8700 Beverly Blvd
City Los Angeles
ZIP/Postal code 90048
Country USA
Platforms (1)
GPL13534 Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482)
Samples (12)
GSM1303510 Fibroblast 83 - parental
GSM1303511 83i-ipsc - P13
GSM1303512 83i-ipsc - P39
BioProject PRJNA233965

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE53918_RAW.tar 183.1 Mb (http)(custom) TAR
GSE53918_signal_iintensities.txt.gz 69.8 Mb (ftp)(http) TXT
Processed data included within Sample table

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