 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Mar 12, 2010 |
| Title |
Gene expression profiles induced by knockdown and overexpression of p63 variants in MCF-10A mammary epithelial cell line |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
p63 is critical for epithelial development yet little is known about the transcriptional programmes it regulates. The p63 transactivating (TA) isoforms contain an amino-terminal exon that encodes a p53-like transactivation domain, whereas ΔN-isoforms lack this domain but contain the common DNA binding domain (DBD), suggesting that TAp63 and ΔNp63 isoforms may have opposing functions. By characterising transcriptional changes and cellular effects following modulation of p63 expression, we have defined a vital role for p63 in cellular adhesion. Knockdown of p63 expression caused downregulation of cell adhesion-associated genes, cell detachment and anoikis in mammary epithelial cells and keratinocytes. Conversely, overexpression of the TAp63γ or ΔNp63α isoforms of p63 upregulated cell adhesion molecules, increased cellular adhesion and conferred resistance to anoikis.
|
| |
|
| Overall design |
Total RNA was isolated 48 h after retroviral or adenoviral infection and was subjected to reverse transcription, labelling and hybridization. The knockdown samples were performed in duplicate with shRNA vector control, shRNA targetting all isoforms of p63 (shDBD) and shRNA targetting p63 isoforms containing the transactivating (TA) domains. The overexpression samples were performed in triplicate with vector control, overexpression of the ΔNp63α isoform, and overexpression of the TAp63γ isoforms.
|
| |
|
| Contributor(s) |
Carroll DK, Carroll JS, Leong C, Chen F, Brown M, Mills AA, Brugge JS, Ellison LW |
| Citation(s) |
16715076, 20194747 |
| |
| Submission date |
Feb 11, 2010 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Joan S. Brugge |
| E-mail(s) |
joan_brugge@hms.harvard.edu
|
| Organization name |
Harvard Medical School
|
| Department |
Department of Cell Biology
|
| Lab |
Brugge
|
| Street address |
240 Longwood Avenue
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
|
| Samples (15)
|
| GSM508612 |
MCF-10A cells, shRNA vector control, biological rep1 |
| GSM508613 |
MCF-10A cells, shRNA vector control, biological rep2 |
| GSM508614 |
MCF-10A cells, shRNA targetting p63 DBD, biological rep1 |
| GSM508615 |
MCF-10A cells, shRNA targetting p63 DBD, biological rep2 |
| GSM508616 |
MCF-10A cells, shRNA targetting p63 TA, biological rep1 |
| GSM508617 |
MCF-10A cells, shRNA targetting p63 TA, biological rep2 |
| GSM508618 |
MCF-10A cells, overexpression vector control, biological rep1 |
| GSM508619 |
MCF-10A cells, overexpression vector control, biological rep2 |
| GSM508620 |
MCF-10A cells, overexpression vector control, biological rep3 |
| GSM508621 |
MCF-10A cells, overexpression ΔNp63α, biological rep1 |
| GSM508622 |
MCF-10A cells, overexpression ΔNp63α, biological rep2 |
| GSM508623 |
MCF-10A cells, overexpression ΔNp63α, biological rep3 |
| GSM508624 |
MCF-10A cells, overexpression TAp63γ, biological rep1 |
| GSM508625 |
MCF-10A cells, overexpression TAp63γ, biological rep2 |
| GSM508626 |
MCF-10A cells, overexpression TAp63γ, biological rep3 |
|
| Relations |
| BioProject |
PRJNA125319 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE20286_RAW.tar |
50.8 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...