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Series GSE20286 Query DataSets for GSE20286
Status Public on Mar 12, 2010
Title Gene expression profiles induced by knockdown and overexpression of p63 variants in MCF-10A mammary epithelial cell line
Organism Homo sapiens
Experiment type Expression profiling by array
Summary p63 is critical for epithelial development yet little is known about the transcriptional programmes it regulates. The p63 transactivating (TA) isoforms contain an amino-terminal exon that encodes a p53-like transactivation domain, whereas ΔN-isoforms lack this domain but contain the common DNA binding domain (DBD), suggesting that TAp63 and ΔNp63 isoforms may have opposing functions. By characterising transcriptional changes and cellular effects following modulation of p63 expression, we have defined a vital role for p63 in cellular adhesion. Knockdown of p63 expression caused downregulation of cell adhesion-associated genes, cell detachment and anoikis in mammary epithelial cells and keratinocytes. Conversely, overexpression of the TAp63γ or ΔNp63α isoforms of p63 upregulated cell adhesion molecules, increased cellular adhesion and conferred resistance to anoikis.
Overall design Total RNA was isolated 48 h after retroviral or adenoviral infection and was subjected to reverse transcription, labelling and hybridization. The knockdown samples were performed in duplicate with shRNA vector control, shRNA targetting all isoforms of p63 (shDBD) and shRNA targetting p63 isoforms containing the transactivating (TA) domains. The overexpression samples were performed in triplicate with vector control, overexpression of the ΔNp63α isoform, and overexpression of the TAp63γ isoforms.
Contributor(s) Carroll DK, Carroll JS, Leong C, Chen F, Brown M, Mills AA, Brugge JS, Ellison LW
Citation(s) 16715076, 20194747
Submission date Feb 11, 2010
Last update date Dec 06, 2018
Contact name Joan S. Brugge
Organization name Harvard Medical School
Department Department of Cell Biology
Lab Brugge
Street address 240 Longwood Avenue
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (15)
GSM508612 MCF-10A cells, shRNA vector control, biological rep1
GSM508613 MCF-10A cells, shRNA vector control, biological rep2
GSM508614 MCF-10A cells, shRNA targetting p63 DBD, biological rep1
BioProject PRJNA125319

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Supplementary file Size Download File type/resource
GSE20286_RAW.tar 50.8 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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