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Sample GSM508613 Query DataSets for GSM508613
Status Public on Mar 12, 2010
Title MCF-10A cells, shRNA vector control, biological rep2
Sample type RNA
Source name MCF-10A cells, shRNA vector control
Organism Homo sapiens
Characteristics cell line: MCF-10A
Treatment protocol Retroviral or adenoviral infection for 48 hours
Growth protocol Standard MCF10A growth conditions
Extracted molecule total RNA
Extraction protocol RNeasy miniprep columns (Qiagen) were used according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNAs were prepared according to the Affymetrix standard labeling protocol.
Hybridization protocol Following fragmentation, 10 ug cRNA were hybridized to the Affymetrix GeneChip Human Genome U133Av2 for 16 hrs. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneArray Chip Scanner, HP/Affymetrix Inc.
Description shRNA_vector2
Data processing Background correction and normalization of microarray data was performed using the Robust Multichip Average method implemented in the rma function of R/BioConductor.
Submission date Feb 11, 2010
Last update date Mar 11, 2010
Contact name Joan S. Brugge
Organization name Harvard Medical School
Department Department of Cell Biology
Lab Brugge
Street address 240 Longwood Avenue
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL571
Series (1)
GSE20286 Gene expression profiles induced by knockdown and overexpression of p63 variants in MCF-10A mammary epithelial cell line

Data table header descriptions
VALUE RMA signal intensity

Data table
1007_s_at 11.4458306
1053_at 8.7484169
117_at 6.350343954
121_at 9.261071553
1255_g_at 4.346612259
1294_at 7.053149744
1316_at 6.631232187
1320_at 5.986167713
1405_i_at 4.337462163
1431_at 4.218091202
1438_at 8.643036823
1487_at 9.91122529
1494_f_at 7.285683644
1598_g_at 10.85991941
160020_at 9.819465459
1729_at 8.966987319
177_at 6.113384966
1773_at 8.145269301
179_at 9.774250944
1861_at 8.223577701

Total number of rows: 22277

Table truncated, full table size 496 Kbytes.

Supplementary file Size Download File type/resource
GSM508613.CEL.gz 3.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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