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Sample GSM508622 Query DataSets for GSM508622
Status Public on Mar 12, 2010
Title MCF-10A cells, overexpression ΔNp63α, biological rep2
Sample type RNA
 
Source name MCF-10A cells, overexpression ΔNp63α
Organism Homo sapiens
Characteristics cell line: MCF-10A
Treatment protocol Retroviral or adenoviral infection for 48 hours
Growth protocol Standard MCF10A growth conditions
Extracted molecule total RNA
Extraction protocol RNeasy miniprep columns (Qiagen) were used according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNAs were prepared according to the Affymetrix standard labeling protocol.
 
Hybridization protocol Following fragmentation, 10 ug cRNA were hybridized to the Affymetrix GeneChip Human Genome U133Av2 for 16 hrs. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneArray Chip Scanner, HP/Affymetrix Inc.
Description Overexpress_ΔNp63α_2
Data processing Background correction and normalization of microarray data was performed using the Robust Multichip Average method implemented in the rma function of R/BioConductor.
 
Submission date Feb 11, 2010
Last update date Mar 11, 2010
Contact name Joan S. Brugge
E-mail(s) joan_brugge@hms.harvard.edu
Organization name Harvard Medical School
Department Department of Cell Biology
Lab Brugge
Street address 240 Longwood Avenue
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL571
Series (1)
GSE20286 Gene expression profiles induced by knockdown and overexpression of p63 variants in MCF-10A mammary epithelial cell line

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
1007_s_at 10.84416594
1053_at 8.509931489
117_at 6.293135798
121_at 8.412904195
1255_g_at 3.828501292
1294_at 7.376216054
1316_at 5.226857995
1320_at 6.020280601
1405_i_at 4.137382538
1431_at 4.088952431
1438_at 6.889095952
1487_at 9.204984174
1494_f_at 6.410237293
1598_g_at 9.891590459
160020_at 8.509183555
1729_at 8.21205808
177_at 5.856942127
1773_at 7.329337242
179_at 9.420162788
1861_at 7.75890757

Total number of rows: 22277

Table truncated, full table size 496 Kbytes.




Supplementary file Size Download File type/resource
GSM508622.CEL.gz 3.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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