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Sample GSM508618 Query DataSets for GSM508618
Status Public on Mar 12, 2010
Title MCF-10A cells, overexpression vector control, biological rep1
Sample type RNA
Source name MCF-10A cells, overexpression vector control
Organism Homo sapiens
Characteristics cell line: MCF-10A
Treatment protocol Retroviral or adenoviral infection for 48 hours
Growth protocol Standard MCF10A growth conditions
Extracted molecule total RNA
Extraction protocol RNeasy miniprep columns (Qiagen) were used according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNAs were prepared according to the Affymetrix standard labeling protocol.
Hybridization protocol Following fragmentation, 10 ug cRNA were hybridized to the Affymetrix GeneChip Human Genome U133Av2 for 16 hrs. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneArray Chip Scanner, HP/Affymetrix Inc.
Description Overexpress_vector1
Data processing Background correction and normalization of microarray data was performed using the Robust Multichip Average method implemented in the rma function of R/BioConductor.
Submission date Feb 11, 2010
Last update date Mar 11, 2010
Contact name Joan S. Brugge
Organization name Harvard Medical School
Department Department of Cell Biology
Lab Brugge
Street address 240 Longwood Avenue
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL571
Series (1)
GSE20286 Gene expression profiles induced by knockdown and overexpression of p63 variants in MCF-10A mammary epithelial cell line

Data table header descriptions
VALUE RMA signal intensity

Data table
1007_s_at 10.43723994
1053_at 8.056261916
117_at 6.127199217
121_at 8.264994707
1255_g_at 3.755198918
1294_at 6.235046763
1316_at 5.18619178
1320_at 5.886705616
1405_i_at 3.873677011
1431_at 3.951183705
1438_at 7.231461792
1487_at 9.415921519
1494_f_at 6.356471383
1598_g_at 9.91431144
160020_at 8.279480929
1729_at 7.875278128
177_at 5.851241768
1773_at 7.041367602
179_at 9.263827491
1861_at 8.310341233

Total number of rows: 22277

Table truncated, full table size 496 Kbytes.

Supplementary file Size Download File type/resource
GSM508618.CEL.gz 3.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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