GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM508624 Query DataSets for GSM508624
Status Public on Mar 12, 2010
Title MCF-10A cells, overexpression TAp63γ, biological rep1
Sample type RNA
Source name MCF-10A cells, overexpression TAp63γ
Organism Homo sapiens
Characteristics cell line: MCF-10A
Treatment protocol Retroviral or adenoviral infection for 48 hours
Growth protocol Standard MCF10A growth conditions
Extracted molecule total RNA
Extraction protocol RNeasy miniprep columns (Qiagen) were used according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNAs were prepared according to the Affymetrix standard labeling protocol.
Hybridization protocol Following fragmentation, 10 ug cRNA were hybridized to the Affymetrix GeneChip Human Genome U133Av2 for 16 hrs. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneArray Chip Scanner, HP/Affymetrix Inc.
Description Overexpress_TAp63γ_1
Data processing Background correction and normalization of microarray data was performed using the Robust Multichip Average method implemented in the rma function of R/BioConductor.
Submission date Feb 11, 2010
Last update date Mar 11, 2010
Contact name Joan S. Brugge
Organization name Harvard Medical School
Department Department of Cell Biology
Lab Brugge
Street address 240 Longwood Avenue
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL571
Series (1)
GSE20286 Gene expression profiles induced by knockdown and overexpression of p63 variants in MCF-10A mammary epithelial cell line

Data table header descriptions
VALUE RMA signal intensity

Data table
1007_s_at 11.80733733
1053_at 8.199374586
117_at 6.65348137
121_at 8.545335253
1255_g_at 3.836395338
1294_at 7.047099598
1316_at 5.148669164
1320_at 6.233395822
1405_i_at 4.497477382
1431_at 4.151189059
1438_at 7.31287328
1487_at 9.766545252
1494_f_at 6.697655562
1598_g_at 10.42525221
160020_at 8.966130788
1729_at 8.723274187
177_at 5.881004438
1773_at 7.667736076
179_at 9.440045649
1861_at 8.104948848

Total number of rows: 22277

Table truncated, full table size 496 Kbytes.

Supplementary file Size Download File type/resource
GSM508624.CEL.gz 3.3 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap