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Sample GSM508620 Query DataSets for GSM508620
Status Public on Mar 12, 2010
Title MCF-10A cells, overexpression vector control, biological rep3
Sample type RNA
 
Source name MCF-10A cells, overexpression vector control
Organism Homo sapiens
Characteristics cell line: MCF-10A
Treatment protocol Retroviral or adenoviral infection for 48 hours
Growth protocol Standard MCF10A growth conditions
Extracted molecule total RNA
Extraction protocol RNeasy miniprep columns (Qiagen) were used according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNAs were prepared according to the Affymetrix standard labeling protocol.
 
Hybridization protocol Following fragmentation, 10 ug cRNA were hybridized to the Affymetrix GeneChip Human Genome U133Av2 for 16 hrs. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneArray Chip Scanner, HP/Affymetrix Inc.
Description Overexpress_vector3
Data processing Background correction and normalization of microarray data was performed using the Robust Multichip Average method implemented in the rma function of R/BioConductor.
 
Submission date Feb 11, 2010
Last update date Mar 11, 2010
Contact name Joan S. Brugge
E-mail(s) joan_brugge@hms.harvard.edu
Organization name Harvard Medical School
Department Department of Cell Biology
Lab Brugge
Street address 240 Longwood Avenue
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL571
Series (1)
GSE20286 Gene expression profiles induced by knockdown and overexpression of p63 variants in MCF-10A mammary epithelial cell line

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
1007_s_at 10.50744995
1053_at 8.130753606
117_at 6.420733536
121_at 8.326817712
1255_g_at 3.815258091
1294_at 6.557367044
1316_at 5.060627024
1320_at 5.985911777
1405_i_at 4.103637437
1431_at 3.971823164
1438_at 7.184566307
1487_at 9.478016959
1494_f_at 6.502470575
1598_g_at 9.891328507
160020_at 8.353090455
1729_at 7.792028659
177_at 5.842161792
1773_at 7.179320378
179_at 9.23405928
1861_at 8.187458144

Total number of rows: 22277

Table truncated, full table size 496 Kbytes.




Supplementary file Size Download File type/resource
GSM508620.CEL.gz 3.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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