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Sample GSM508616 Query DataSets for GSM508616
Status Public on Mar 12, 2010
Title MCF-10A cells, shRNA targetting p63 TA, biological rep1
Sample type RNA
 
Source name MCF-10A cells, shRNA targetting p63 TA
Organism Homo sapiens
Characteristics cell line: MCF-10A
Treatment protocol Retroviral or adenoviral infection for 48 hours
Growth protocol Standard MCF10A growth conditions
Extracted molecule total RNA
Extraction protocol RNeasy miniprep columns (Qiagen) were used according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNAs were prepared according to the Affymetrix standard labeling protocol.
 
Hybridization protocol Following fragmentation, 10 ug cRNA were hybridized to the Affymetrix GeneChip Human Genome U133Av2 for 16 hrs. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneArray Chip Scanner, HP/Affymetrix Inc.
Description shTA1
Data processing Background correction and normalization of microarray data was performed using the Robust Multichip Average method implemented in the rma function of R/BioConductor.
 
Submission date Feb 11, 2010
Last update date Mar 11, 2010
Contact name Joan S. Brugge
E-mail(s) joan_brugge@hms.harvard.edu
Organization name Harvard Medical School
Department Department of Cell Biology
Lab Brugge
Street address 240 Longwood Avenue
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL571
Series (1)
GSE20286 Gene expression profiles induced by knockdown and overexpression of p63 variants in MCF-10A mammary epithelial cell line

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
1007_s_at 11.46300693
1053_at 8.657251737
117_at 6.352528812
121_at 9.245321814
1255_g_at 4.51223333
1294_at 7.05044767
1316_at 6.638530358
1320_at 5.947143491
1405_i_at 4.30491222
1431_at 4.299206975
1438_at 8.662417191
1487_at 10.07119899
1494_f_at 7.125031317
1598_g_at 10.57573334
160020_at 9.565198242
1729_at 8.841608705
177_at 6.132091626
1773_at 7.998684287
179_at 9.77252563
1861_at 7.715842698

Total number of rows: 22277

Table truncated, full table size 496 Kbytes.




Supplementary file Size Download File type/resource
GSM508616.CEL.gz 3.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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