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Sample GSM508612 Query DataSets for GSM508612
Status Public on Mar 12, 2010
Title MCF-10A cells, shRNA vector control, biological rep1
Sample type RNA
 
Source name MCF-10A cells, shRNA vector control
Organism Homo sapiens
Characteristics cell line: MCF-10A
Treatment protocol Retroviral or adenoviral infection for 48 hours
Growth protocol Standard MCF10A growth conditions
Extracted molecule total RNA
Extraction protocol RNeasy miniprep columns (Qiagen) were used according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNAs were prepared according to the Affymetrix standard labeling protocol.
 
Hybridization protocol Following fragmentation, 10 ug cRNA were hybridized to the Affymetrix GeneChip Human Genome U133Av2 for 16 hrs. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneArray Chip Scanner, HP/Affymetrix Inc.
Description shRNA_vector1
Data processing Background correction and normalization of microarray data was performed using the Robust Multichip Average method implemented in the rma function of R/BioConductor.
 
Submission date Feb 11, 2010
Last update date Mar 11, 2010
Contact name Joan S. Brugge
E-mail(s) joan_brugge@hms.harvard.edu
Organization name Harvard Medical School
Department Department of Cell Biology
Lab Brugge
Street address 240 Longwood Avenue
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL571
Series (1)
GSE20286 Gene expression profiles induced by knockdown and overexpression of p63 variants in MCF-10A mammary epithelial cell line

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
1007_s_at 11.46014177
1053_at 8.676517706
117_at 6.451843566
121_at 9.34251907
1255_g_at 4.488851618
1294_at 7.130461704
1316_at 6.656980601
1320_at 6.22063516
1405_i_at 4.418098324
1431_at 4.238446129
1438_at 8.638299288
1487_at 9.951668304
1494_f_at 7.378959387
1598_g_at 10.93787296
160020_at 9.836403566
1729_at 9.012927205
177_at 6.000779972
1773_at 8.118625392
179_at 9.857161356
1861_at 8.121480671

Total number of rows: 22277

Table truncated, full table size 496 Kbytes.




Supplementary file Size Download File type/resource
GSM508612.CEL.gz 3.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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