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Sample GSM508614 Query DataSets for GSM508614
Status Public on Mar 12, 2010
Title MCF-10A cells, shRNA targetting p63 DBD, biological rep1
Sample type RNA
Source name MCF-10A cells, shRNA targetting p63 DBD
Organism Homo sapiens
Characteristics cell line: MCF-10A
Treatment protocol Retroviral or adenoviral infection for 48 hours
Growth protocol Standard MCF10A growth conditions
Extracted molecule total RNA
Extraction protocol RNeasy miniprep columns (Qiagen) were used according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNAs were prepared according to the Affymetrix standard labeling protocol.
Hybridization protocol Following fragmentation, 10 ug cRNA were hybridized to the Affymetrix GeneChip Human Genome U133Av2 for 16 hrs. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneArray Chip Scanner, HP/Affymetrix Inc.
Description shDBD1
Data processing Background correction and normalization of microarray data was performed using the Robust Multichip Average method implemented in the rma function of R/BioConductor.
Submission date Feb 11, 2010
Last update date Mar 11, 2010
Contact name Joan S. Brugge
Organization name Harvard Medical School
Department Department of Cell Biology
Lab Brugge
Street address 240 Longwood Avenue
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL571
Series (1)
GSE20286 Gene expression profiles induced by knockdown and overexpression of p63 variants in MCF-10A mammary epithelial cell line

Data table header descriptions
VALUE RMA signal intensity

Data table
1007_s_at 11.04658949
1053_at 7.82127654
117_at 6.370069857
121_at 9.224372174
1255_g_at 4.495108072
1294_at 7.413462776
1316_at 6.658195749
1320_at 6.202813152
1405_i_at 4.277497073
1431_at 4.356491855
1438_at 8.107795425
1487_at 10.15564387
1494_f_at 7.444068669
1598_g_at 10.70545797
160020_at 9.420874618
1729_at 8.92696043
177_at 6.171891475
1773_at 7.890112107
179_at 9.879564696
1861_at 8.535836929

Total number of rows: 22277

Table truncated, full table size 496 Kbytes.

Supplementary file Size Download File type/resource
GSM508614.CEL.gz 3.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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