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Sample GSM508617 Query DataSets for GSM508617
Status Public on Mar 12, 2010
Title MCF-10A cells, shRNA targetting p63 TA, biological rep2
Sample type RNA
 
Source name MCF-10A cells, shRNA targetting p63 TA
Organism Homo sapiens
Characteristics cell line: MCF-10A
Treatment protocol Retroviral or adenoviral infection for 48 hours
Growth protocol Standard MCF10A growth conditions
Extracted molecule total RNA
Extraction protocol RNeasy miniprep columns (Qiagen) were used according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNAs were prepared according to the Affymetrix standard labeling protocol.
 
Hybridization protocol Following fragmentation, 10 ug cRNA were hybridized to the Affymetrix GeneChip Human Genome U133Av2 for 16 hrs. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneArray Chip Scanner, HP/Affymetrix Inc.
Description shTA2
Data processing Background correction and normalization of microarray data was performed using the Robust Multichip Average method implemented in the rma function of R/BioConductor.
 
Submission date Feb 11, 2010
Last update date Mar 11, 2010
Contact name Joan S. Brugge
E-mail(s) joan_brugge@hms.harvard.edu
Organization name Harvard Medical School
Department Department of Cell Biology
Lab Brugge
Street address 240 Longwood Avenue
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL571
Series (1)
GSE20286 Gene expression profiles induced by knockdown and overexpression of p63 variants in MCF-10A mammary epithelial cell line

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
1007_s_at 11.32353616
1053_at 8.794268788
117_at 6.393024656
121_at 9.243754863
1255_g_at 4.405268289
1294_at 6.986824062
1316_at 6.703144515
1320_at 5.966375086
1405_i_at 4.190734645
1431_at 4.26455057
1438_at 8.432300233
1487_at 9.867228311
1494_f_at 7.375274609
1598_g_at 10.58284347
160020_at 9.454002537
1729_at 8.910993609
177_at 6.011542422
1773_at 8.057315085
179_at 9.769931844
1861_at 7.777971074

Total number of rows: 22277

Table truncated, full table size 496 Kbytes.




Supplementary file Size Download File type/resource
GSM508617.CEL.gz 3.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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