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Sample GSM508615 Query DataSets for GSM508615
Status Public on Mar 12, 2010
Title MCF-10A cells, shRNA targetting p63 DBD, biological rep2
Sample type RNA
Source name MCF-10A cells, shRNA targetting p63 DBD
Organism Homo sapiens
Characteristics cell line: MCF-10A
Treatment protocol Retroviral or adenoviral infection for 48 hours
Growth protocol Standard MCF10A growth conditions
Extracted molecule total RNA
Extraction protocol RNeasy miniprep columns (Qiagen) were used according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNAs were prepared according to the Affymetrix standard labeling protocol.
Hybridization protocol Following fragmentation, 10 ug cRNA were hybridized to the Affymetrix GeneChip Human Genome U133Av2 for 16 hrs. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneArray Chip Scanner, HP/Affymetrix Inc.
Description shDBD2
Data processing Background correction and normalization of microarray data was performed using the Robust Multichip Average method implemented in the rma function of R/BioConductor.
Submission date Feb 11, 2010
Last update date Mar 11, 2010
Contact name Joan S. Brugge
Organization name Harvard Medical School
Department Department of Cell Biology
Lab Brugge
Street address 240 Longwood Avenue
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL571
Series (1)
GSE20286 Gene expression profiles induced by knockdown and overexpression of p63 variants in MCF-10A mammary epithelial cell line

Data table header descriptions
VALUE RMA signal intensity

Data table
1007_s_at 11.05952424
1053_at 7.901350303
117_at 6.356196287
121_at 9.183135094
1255_g_at 4.509467758
1294_at 7.318950492
1316_at 6.613535922
1320_at 6.120550078
1405_i_at 4.38600951
1431_at 4.28881121
1438_at 8.107770416
1487_at 10.04800066
1494_f_at 7.384176968
1598_g_at 10.5847805
160020_at 9.393733306
1729_at 8.930400643
177_at 6.128720965
1773_at 7.907090355
179_at 9.874567151
1861_at 8.423826863

Total number of rows: 22277

Table truncated, full table size 496 Kbytes.

Supplementary file Size Download File type/resource
GSM508615.CEL.gz 3.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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