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Links from GEO DataSets

Items: 20

1.
Full record GDS2770

Estrogen effect on breast cancer cell line coexpressing estrogen receptors alpha and beta

Analysis of estrogen receptor (ER) alpha positive MCF-7 breast cancer cells following introduction of ERbeta and treatment with estrogen. The majority of breast cancers express both ERalpha and ERbeta. Results provide insight into the comodulatory effects of these two ERs.
Organism:
Homo sapiens
Type:
Expression profiling by array, count, 2 agent, 3 infection sets
Platform:
GPL96
Series:
GSE4006
12 Samples
Download data
DataSet
Accession:
GDS2770
ID:
2770
2.

Estrogen effects on MCF-7 breast cancer cells co-expressing ERa and ERb

(Submitter supplied) Two subtypes of the estrogen receptor, ERalpha and ERbeta, mediate the actions of estrogens, and the majority of human breast tumors contain both ERalpha and ERbeta. To examine the possible interactions and modulatory effects of ERbeta on ERalpha activity, we have used adenoviral gene delivery to produce human breast cancer (MCF-7) cells expressing ERbeta, along with their endogenous ERalpha. We have examined the effects of ERβ expression on genome-wide gene expression by Affymetrix GeneChip microarrays. more...
Organism:
Homo sapiens
Type:
Expression profiling by array
Dataset:
GDS2770
Platform:
GPL96
12 Samples
Download data
Series
Accession:
GSE4006
ID:
200004006
3.

U2OS-ERa, U2OS-ERb, and U2OS-ERab cells treated with 4HT or E2

(Submitter supplied) U2OS-ERa, U2OS-ERb, and U2OS-ERab cells treated with 4HT or E2 Keywords: other
Organism:
Homo sapiens
Type:
Expression profiling by array
Dataset:
GDS1094
Platform:
GPL201
45 Samples
Download data: CEL
Series
Accession:
GSE2292
ID:
200002292
4.
Full record GDS1094

Estrogen receptor alpha/beta heterodimer action in response to estrogen and tamoxifen

Expression profiling of U2OS osteosarcoma cell line expressing estrogen receptor/alpha heterodimer after treatment with 10 nM 17beta-estradiol (E2) or 10 nM 4-OH tamoxifen (4HT) for 24 hours. Results provide insight into the role of estrogen receptor/alpha heterodimer in gene regulation.
Organism:
Homo sapiens
Type:
Expression profiling by array, count, 3 agent, 3 genotype/variation sets
Platform:
GPL201
Series:
GSE2292
45 Samples
Download data: CEL
DataSet
Accession:
GDS1094
ID:
1094
5.

Expression data from human breast cancer cells (MCF-7) coexpressing ERalpha and Erbeta, treated with phytoestrogens

(Submitter supplied) We used microarrays to detail the global transcriptional response mediated by ERalpha or ERbeta to the phytoestrogen genistein in the MCF-7 human breast cancer cell model. Keywords: ligand response over time course
Organism:
Homo sapiens
Type:
Expression profiling by array
Platform:
GPL96
105 Samples
Download data: CEL
Series
Accession:
GSE9936
ID:
200009936
6.

Estrogen-regulated gene expression in human osteosarcoma cells stably transfected with estrogen receptor alpha or beta

(Submitter supplied) Estrogens exert many important effects in bone, a tissue that contains both estrogen receptors alpha and beta (ERalpha and ERbeta). To compare the actions of these receptors, we generated U2OS human osteosarcoma cells stably expressing ERalpha or ERbeta, at levels comparable to those in osteoblasts, and we characterized their response to estradiol (E2) over time using Affymetrix GeneChip microarrays to determine the expression of approximately 12,000 genes, followed by quantitative PCR verification of the regulation of selected genes. more...
Organism:
Homo sapiens
Type:
Expression profiling by array
Dataset:
GDS884
Platform:
GPL91
20 Samples
Download data
Series
Accession:
GSE1153
ID:
200001153
7.
Full record GDS884

Estradiol effect on osteosarcoma cells expressing estrogen receptor alpha or beta: time course

Expression profiling of U2OS osteosarcoma cells after treatment with 17beta-estradiol (E2) for various lengths of time up to 48 hours. U2OS transfected with either estrogen receptor (ER) alpha or beta. Results identify signaling pathways in bone regulated by E2 through ERalpha and ERbeta.
Organism:
Homo sapiens
Type:
Expression profiling by array, count, 2 agent, 2 cell line, 5 time sets
Platform:
GPL91
Series:
GSE1153
20 Samples
Download data
DataSet
Accession:
GDS884
ID:
884
8.

Anti-prolif. effect of E2 in breast cancer cells that re-exp. ERalpha is mediated by aberr. regulat. of cell cycle genes

(Submitter supplied) Gene expression changes caused by estrogen treatment of breast cancer cells that re-express ERalpha was investigated by infecting ER-negative MDA-MB-231 breast cancer cells for 24 h with recombinant adenovirus encoding full-length human ERalpha (Ad-ERalpha) or control vector (Ad-LacZ), and treating them with 0·01% ethanol (vehicle control) or 10-8 M 17beta-estradiol (E2). After 48 h of treatment, total RNA was isolated and used for transcript profiling on Affymetrix GeneChips. more...
Organism:
Homo sapiens
Type:
Expression profiling by array
Dataset:
GDS1326
Platform:
GPL96
12 Samples
Download data
Series
Accession:
GSE2251
ID:
200002251
9.
Full record GDS1326

Breast cancer cells reexpressing estrogen receptor alpha response to 17beta-estradiol

Analysis of the response of estrogen receptor (ER) negative MDA-MB-231 breast cancer cells infected with full-length ER alpha adenoviral constructs to treatment with 17beta-estradiol (E2). Results provide insight into the anti-proliferative effect of E2 on breast cancer cells reexpressing ER.
Organism:
Homo sapiens
Type:
Expression profiling by array, count, 2 agent, 2 protocol sets
Platform:
GPL96
Series:
GSE2251
12 Samples
Download data
DataSet
Accession:
GDS1326
ID:
1326
10.

Overlapping and distinct functions between ERα and ERβ homodimers and corresponding transcriptomes in the same cellular context

(Submitter supplied) The two estrogen receptors, ERα and ERβ function as ligand-inducible transcription factors. Most in vitro studies have reported that ERα drives breast cancer growth whereas ERβ, if expressed, suppresses growth. To dissect function and gene expression profile regulated by ERα or ERβ, respectively, we generated a novel cell model expressing only ERβ, by applying CRISPR-cas9 to delete ERα in MCF7 cells with stable Tet-Off-inducible ERβ expression. more...
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL21697
12 Samples
Download data: XLSX
11.

[E-MTAB-345] ChIP-Seq of human MCF-7 cells with anti-ERalpha following estrogen treatment

(Submitter supplied) We performed chromatin immunoprecipitation (ChIP) with overnight with antibodies against the C-terminus (HC-20, from Santa Cruz Biotechnology, Europe) or the N-terminus (anti-Estrogen Receptor 18-32, from SigmaAldrich, Italy) of human ER-alpha with or without estrogen treatment for 45 minutes on TAP-ER-beta cells, and the immunoprecipitated DNA was sequenced with the Illumina/Solexa Genome Analyzer. more...
Organism:
Homo sapiens
Type:
Genome binding/occupancy profiling by high throughput sequencing
Platform:
GPL9115
8 Samples
Download data: TXT
Series
Accession:
GSE25769
ID:
200025769
12.

Gene expression profiling in T-47D breast cancer cells following inducible expression of estrogen receptor beta

(Submitter supplied) T-47D breast cancer cells were stably transfected with a tet-off inducible construct encoding estrogen receptor beta. Microarray experiments were carried out at multiple time points 1-30 hours following treatment with estradiol and under induction and non-induction conditions. Keywords: Inducible expression of ERbeta
Organism:
Homo sapiens
Type:
Expression profiling by array
Platform:
GPL4840
20 Samples
Download data: GPR
Series
Accession:
GSE7206
ID:
200007206
13.

RNA-seq data from MCF-7 cells (breast cancer model) treated with soy extracts

(Submitter supplied) The MCF-7 were infected with either control adenovirus expressing B-galactosidase (Ad) or adenovirus expressing ERB (AdERbeta) for 72 h. For knockdown of the endogenous ERa in MCF-7 cells, cells were treated with siRNA for 24h (AdERbeta+SiERalpha). Then cells were treated with Veh (0.1% EtOH), 10 nM E2 or 1 uM BEs (botanical extracts) for 24h.
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL11154
30 Samples
Download data: XLSX
14.

Estrogen receptors alpha and beta mediation of gene expression in mouse vascular tissue

(Submitter supplied) Estrogen plays an important role in the regulation of vascular tone and in the pathophysiology of cardiovascular disease. Physiological effects of estrogen are mediated through estrogen receptors alpha (ERalpha) and beta (ERbeta), which are both expressed in vascular smooth muscle and endothelial cells. However, the molecular pathways mediating estrogen effects in blood vessels are not well defined. more...
Organism:
Mus musculus
Type:
Expression profiling by array
Dataset:
GDS3064
Platform:
GPL1261
22 Samples
Download data: CEL, TXT
Series
Accession:
GSE9371
ID:
200009371
15.
Full record GDS3064

Estrogen effect on estrogen receptors alpha and beta single knockouts: aorta

Analysis of estrogen receptor alpha or beta null aortas of ovariectomized animals treated with estrogen. Estrogen is important in vascular tone regulation and in cardiovascular disease pathophysiology. Results provide insight into the mechanisms mediating the effects of estrogen in blood vessels.
Organism:
Mus musculus
Type:
Expression profiling by array, transformed count, 2 agent, 3 genotype/variation sets
Platform:
GPL1261
Series:
GSE9371
22 Samples
Download data: CEL
DataSet
Accession:
GDS3064
ID:
3064
16.

Gene expression profiles elicited by estradiol and endoxifen in MCF7 parental and ER-beta expressing breast cancer cells

(Submitter supplied) We have previously demonstrated that endoxifen is the most important tamoxifen metabolite responsible for eliciting the anti-estrogenic effects of this drug in breast cancer cells expressing estrogen receptor-alpha. However, the relevance of estrogen receptor-beta in mediating endoxifen action has yet to be explored. Therefore, the goals of this study were to determine the differences in the global gene expression profiles elicited by estradiol treatment and endoxifen between parental MCF7 breast cancer cells (expressing estrogen receptor alpha only) and MCF7 cells stably expressing estrogen receptor beta.
Organism:
Homo sapiens
Type:
Expression profiling by array
Platform:
GPL6947
12 Samples
Download data: IDAT, TXT
Series
Accession:
GSE27375
ID:
200027375
17.

Estrogen Receptor Beta Impacts Hormone-Induced Alternative mRNA Splicing in Breast Cancer Cells

(Submitter supplied) Estrogens play an important role in breast cancer (BC) development and progression, where the two isoforms of the estrogen receptor (ERα and ERβ) are generally co-expressed and mediate the effects of these hormones in cancer cells. ERβ has been suggested to exert an antagonist role toward the oncogenic activities of ERα, and for this reason it is considered an oncosuppressor. As clinical evidence regarding a prognostic role for this receptor subtype in hormone-responsive BC is still limited and conflicting, more knowledge is required on the biological functions of ERβ in cancer cells. more...
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL18460
18 Samples
Download data: TXT
18.

ERα gene expression profile in MCF7 cells

(Submitter supplied) This submission is a part of two separate studies: a study of estrogen receptor-alpha (ERalpha)-mediated gene expression in response to different ligands and a study examining the roles of ERalpha and ERbeta in gene regulation in breast cancer cells.
Organism:
Homo sapiens
Type:
Expression profiling by array
Platform:
GPL15127
2 Samples
Download data: GPR
Series
Accession:
GSE45557
ID:
200045557
19.

Support of a bi-faceted role of ERβ in ERα-positive breast cancer cells

(Submitter supplied) Comparison of the basal and estrogen-induced effects on genome-wide transcription in ERα-positive breast cancer cell lines T47D and MCF7 after lentiviral transduction with ERβ.
Organism:
Homo sapiens
Type:
Expression profiling by array
Platform:
GPL15127
8 Samples
Download data: GPR
Series
Accession:
GSE45047
ID:
200045047
20.

Nuclear and Extranuclear Pathway Inputs in the Regulation of Global Gene Expression by Estrogen Receptors

(Submitter supplied) Whereas estrogens exert their effects by binding to nuclear estrogen receptors and directly altering target gene transcription, they can also initiate extranuclear signaling through activation of kinase cascades. We have investigated the impact of estrogen-mediated extranuclear initiated pathways on global gene expression by using estrpgen-dendrimer conjugates. Keywords: ligand treatment
Organism:
Homo sapiens
Type:
Expression profiling by array
Platform:
GPL96
9 Samples
Download data: CEL
Series
Accession:
GSE15717
ID:
200015717
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