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Series GSE9936 Query DataSets for GSE9936
Status Public on Feb 07, 2008
Title Expression data from human breast cancer cells (MCF-7) coexpressing ERalpha and Erbeta, treated with phytoestrogens
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We used microarrays to detail the global transcriptional response mediated by ERalpha or ERbeta to the phytoestrogen genistein in the MCF-7 human breast cancer cell model.
Keywords: ligand response over time course
 
Overall design MCF-7 human breast cancer cells expressing endogenouse Estrogen Receptor Alpha (ERalpha) were infected with adenovirus carrying either estrogen receptor beta (AdERb) or no insert (Ad) at multiplicity of infection (moi) of 20. Cells were then treated with either vehicle control (veh), 6nM 17beta-estradiol (E2), 6nM genistein (LG), 300nM genistein (HG), 300nM S-Equol (EQ), HG+3uM ICI182,780 (IG), EQ+3uM ICI 182,780(IE) for additional periods of 4h or 24hr before RNA extraction and hybridization on Affymetrix microarrays. We sought to determine if genistein and S-Equol, phytoestrogens selective for the ERbeta can elicit transcriptional response distinctive from those mediated by the ERalpha.
 
Contributor(s) Chang EC, Katzenellenbogen BS, Charn TH, Katzenellenbogen JA, Park S, Helferich WG, Komm B
Citation(s) 18258689
Submission date Dec 18, 2007
Last update date Aug 10, 2018
Contact name Edmund C Chang
Organization name Kite Pharma
Street address 2225 Colorado Ave
City Santa Monica
State/province California
ZIP/Postal code 90404
Country USA
 
Platforms (1)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
Samples (105)
GSM251354 Ad+veh for 4h, biological rep1
GSM251355 Ad+veh for 4h, biological rep2
GSM251356 Ad+veh for 4h, biological rep3
Relations
BioProject PRJNA103937

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Supplementary file Size Download File type/resource
GSE9936_RAW.tar 301.4 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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