Similar studies for GEO DataSets (Select 200001153) - GEO DataSets

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Items: 20

Select item 2000011531.

Estrogen-regulated gene expression in human osteosarcoma cells stably transfected with estrogen receptor alpha or beta

(Submitter supplied) Estrogens exert many important effects in bone, a tissue that contains both estrogen receptors alpha and beta (ERalpha and ERbeta). To compare the actions of these receptors, we generated U2OS human osteosarcoma cells stably expressing ERalpha or ERbeta, at levels comparable to those in osteoblasts, and we characterized their response to estradiol (E2) over time using Affymetrix GeneChip microarrays to determine the expression of approximately 12,000 genes, followed by quantitative PCR verification of the regulation of selected genes. more...

Organism:: Homo sapiens

Type:: Expression profiling by array

Dataset:: GDS884
Platform:: GPL91
20 Samples

Download data

Series

Accession:: GSE1153

ID:: 200001153

Select item 8842.

Estradiol effect on osteosarcoma cells expressing estrogen receptor alpha or beta: time course

Expression profiling of U2OS osteosarcoma cells after treatment with 17beta-estradiol (E2) for various lengths of time up to 48 hours. U2OS transfected with either estrogen receptor (ER) alpha or beta. Results identify signaling pathways in bone regulated by E2 through ERalpha and ERbeta.

Organism:: Homo sapiens

Type:: Expression profiling by array, count, 2 agent, 2 cell line, 5 time sets

Platform:: GPL91
Series:: GSE1153
20 Samples

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DataSet

Accession:: GDS884

ID:: 884

Select item 2000022923.

U2OS-ERa, U2OS-ERb, and U2OS-ERab cells treated with 4HT or E2

(Submitter supplied) U2OS-ERa, U2OS-ERb, and U2OS-ERab cells treated with 4HT or E2 Keywords: other

Organism:: Homo sapiens

Type:: Expression profiling by array

Dataset:: GDS1094
Platform:: GPL201
45 Samples

Download data: CEL

Series

Accession:: GSE2292

ID:: 200002292

Select item 10944.

Estrogen receptor alpha/beta heterodimer action in response to estrogen and tamoxifen

Expression profiling of U2OS osteosarcoma cell line expressing estrogen receptor/alpha heterodimer after treatment with 10 nM 17beta-estradiol (E2) or 10 nM 4-OH tamoxifen (4HT) for 24 hours. Results provide insight into the role of estrogen receptor/alpha heterodimer in gene regulation.

Organism:: Homo sapiens

Type:: Expression profiling by array, count, 3 agent, 3 genotype/variation sets

Platform:: GPL201
Series:: GSE2292
45 Samples

Download data: CEL

DataSet

Accession:: GDS1094

ID:: 1094

Select item 2000040065.

Estrogen effects on MCF-7 breast cancer cells co-expressing ERa and ERb

(Submitter supplied) Two subtypes of the estrogen receptor, ERalpha and ERbeta, mediate the actions of estrogens, and the majority of human breast tumors contain both ERalpha and ERbeta. To examine the possible interactions and modulatory effects of ERbeta on ERalpha activity, we have used adenoviral gene delivery to produce human breast cancer (MCF-7) cells expressing ERbeta, along with their endogenous ERalpha. We have examined the effects of ERβ expression on genome-wide gene expression by Affymetrix GeneChip microarrays. more...

Organism:: Homo sapiens

Type:: Expression profiling by array

Dataset:: GDS2770
Platform:: GPL96
12 Samples

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Series

Accession:: GSE4006

ID:: 200004006

Select item 27706.

Estrogen effect on breast cancer cell line coexpressing estrogen receptors alpha and beta

Analysis of estrogen receptor (ER) alpha positive MCF-7 breast cancer cells following introduction of ERbeta and treatment with estrogen. The majority of breast cancers express both ERalpha and ERbeta. Results provide insight into the comodulatory effects of these two ERs.

Organism:: Homo sapiens

Type:: Expression profiling by array, count, 2 agent, 3 infection sets

Platform:: GPL96
Series:: GSE4006
12 Samples

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DataSet

Accession:: GDS2770

ID:: 2770

Select item 2000560667.

RNA-seq data from MCF-7 cells (breast cancer model) treated with soy extracts

(Submitter supplied) The MCF-7 were infected with either control adenovirus expressing B-galactosidase (Ad) or adenovirus expressing ERB (AdERbeta) for 72 h. For knockdown of the endogenous ERa in MCF-7 cells, cells were treated with siRNA for 24h (AdERbeta+SiERalpha). Then cells were treated with Veh (0.1% EtOH), 10 nM E2 or 1 uM BEs (botanical extracts) for 24h.

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL11154
30 Samples

Download data: XLSX

Series

Accession:: GSE56066

ID:: 200056066

PubMed Full text in PMC Similar studies Analyze with GEO2R SRA Run Selector

Select item 2001824318.

Overlapping and distinct functions between ERα and ERβ homodimers and corresponding transcriptomes in the same cellular context

(Submitter supplied) The two estrogen receptors, ERα and ERβ function as ligand-inducible transcription factors. Most in vitro studies have reported that ERα drives breast cancer growth whereas ERβ, if expressed, suppresses growth. To dissect function and gene expression profile regulated by ERα or ERβ, respectively, we generated a novel cell model expressing only ERβ, by applying CRISPR-cas9 to delete ERα in MCF7 cells with stable Tet-Off-inducible ERβ expression. more...

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL21697
12 Samples

Download data: XLSX

Series

Accession:: GSE182431

ID:: 200182431

PubMed Full text in PMC Similar studies Analyze with GEO2R SRA Run Selector

Select item 2000099369.

Expression data from human breast cancer cells (MCF-7) coexpressing ERalpha and Erbeta, treated with phytoestrogens

(Submitter supplied) We used microarrays to detail the global transcriptional response mediated by ERalpha or ERbeta to the phytoestrogen genistein in the MCF-7 human breast cancer cell model. Keywords: ligand response over time course

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL96
105 Samples

Download data: CEL

Series

Accession:: GSE9936

ID:: 200009936

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20001111510.

Selective estrogen receptor-beta agonists repress transcription of proinflammatory

(Submitter supplied) In addition to their role in the development and function of the reproductive system, estrogens have significant anti-inflammatory properties. Although both estrogen receptors (ERs) can mediate anti-inflammatory actions, ERbeta is a more desirable therapeutic target because ERalpha mediates the proliferative effects of estrogens on the mammary gland and uterus. In fact, selective ERbeta agonists have beneficial effects in preclinical models involving inflammation without causing growth-promoting effects on the uterus or mammary gland. more...

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL96
11 Samples

Download data: CEL, CHP

Series

Accession:: GSE11115

ID:: 200011115

Select item 20000466411.

24 hour time course: regulation of uterine genes by estradiol in ovariectomized mice

(Submitter supplied) Using microarray technology, we compared the global expression pattern of uterine RNA from ovariectomized control mice to those of ovariectomized mice treated with estradiol for various intervals between 30 minutes and 24 hours. Keywords: estrogen, uterus, genomic, mouse

Organism:: Mus musculus

Type:: Expression profiling by array

Dataset:: GDS2208
Platform:: GPL891
10 Samples

Download data: TIFF, TXT

Series

Accession:: GSE4664

ID:: 200004664

Select item 220812.

Estradiol effect on the uterus: time course

Expression profiling of uteri from ovariectomized C57BL/6 animals at various time points up to 24 hours following treatment with estradiol (E2). E2 plays a critical role in regulating the growth, differentiation, and secretory function of the uterus.

Organism:: Mus musculus

Type:: Expression profiling by array, log10 ratio, 5 time sets

Platform:: GPL891
Series:: GSE4664
10 Samples

Download data: TIFF, TXT

DataSet

Accession:: GDS2208

ID:: 2208

Select item 20000937113.

Estrogen receptors alpha and beta mediation of gene expression in mouse vascular tissue

(Submitter supplied) Estrogen plays an important role in the regulation of vascular tone and in the pathophysiology of cardiovascular disease. Physiological effects of estrogen are mediated through estrogen receptors alpha (ERalpha) and beta (ERbeta), which are both expressed in vascular smooth muscle and endothelial cells. However, the molecular pathways mediating estrogen effects in blood vessels are not well defined. more...

Organism:: Mus musculus

Type:: Expression profiling by array

Dataset:: GDS3064
Platform:: GPL1261
22 Samples

Download data: CEL, TXT

Series

Accession:: GSE9371

ID:: 200009371

Select item 306414.

Estrogen effect on estrogen receptors alpha and beta single knockouts: aorta

Analysis of estrogen receptor alpha or beta null aortas of ovariectomized animals treated with estrogen. Estrogen is important in vascular tone regulation and in cardiovascular disease pathophysiology. Results provide insight into the mechanisms mediating the effects of estrogen in blood vessels.

Organism:: Mus musculus

Type:: Expression profiling by array, transformed count, 2 agent, 3 genotype/variation sets

Platform:: GPL1261
Series:: GSE9371
22 Samples

Download data: CEL

DataSet

Accession:: GDS3064

ID:: 3064

Select item 20002576915.

[E-MTAB-345] ChIP-Seq of human MCF-7 cells with anti-ERalpha following estrogen treatment

(Submitter supplied) We performed chromatin immunoprecipitation (ChIP) with overnight with antibodies against the C-terminus (HC-20, from Santa Cruz Biotechnology, Europe) or the N-terminus (anti-Estrogen Receptor 18-32, from SigmaAldrich, Italy) of human ER-alpha with or without estrogen treatment for 45 minutes on TAP-ER-beta cells, and the immunoprecipitated DNA was sequenced with the Illumina/Solexa Genome Analyzer. more...

Organism:: Homo sapiens

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL9115
8 Samples

Download data: TXT

Series

Accession:: GSE25769

ID:: 200025769

PubMed Full text in PMC Similar studies

Select item 20006747516.

Transcriptional changes in response to 17β-estradiol, cigarette sidestream smoke particulates, and TNFα treatments in ERα-induced and uninduced CL1-5(TO-ERα) cells

(Submitter supplied) This microarray analysis was designed to determine (1) the impact of ERα expression on cellular TNFα response and estrogen-TNFα signaling crosstalk and (2) whether cigarette sidestream smoke particulates had estrogen-like action in human lung adenocarcinoma cells. The lung adenocarcinoma cell line CL1-5(TO-ERα)#18 was used as a model. Expression of ERα in this cell line is under Tet-on regulation and can be induced by addition of doxycycline. more...

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL10558
18 Samples

Download data: TXT

Series

Accession:: GSE67475

ID:: 200067475

Select item 20002179017.

Genome wide analysis of ER beta in U2OS cells

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Homo sapiens

Type:: Expression profiling by array; Genome binding/occupancy profiling by high throughput sequencing

Platforms:: GPL6884 GPL9052
22 Samples

Download data: BED, TXT

Series

Accession:: GSE21790

ID:: 200021790

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20002177018.

Genome-wide maps of ERbeta binding sites in U2OS cells

(Submitter supplied) Estrogen receptor beta (ERbeta) has potent anti-proliferative and anti-inflammatory properties, suggesting that ER beta-selective agonists might be a new class of therapeutic and chemopreventative agents. To understand how ER beta regulates genes, we identified genes regulated by the unliganded and liganded forms of ER alpha and ER beta in U2OS cells. Microarray data demonstrated that virtually no gene regulation occurred with unliganded ER alpha, whereas many genes were regulated by estradiol (E2). more...