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| Status |
Public on Mar 11, 2015 |
| Title |
Dissection of estrogen receptor alpha signaling pathways in osteoblasts using RNA-sequencing |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The goal is to identify estrogen-dependent gene expression patterns using RNAseq for 3 different ERα forms in human osteoblasts.
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| Overall design |
We analyzed 3 different forms of estrogen receptor alpha (ERα), 1) Wild-type, 2) NERKI (DNA-binding mutant) and 3) NOER (Nuclear Only ERα, not membrane). Each was infected into hFOB (human osteoblast) cells and treated with either vehicle or E2 (10nM) (n=3 for each group/treatment). RNAseq was then performed.
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| Contributor(s) |
Monroe DG, Khosla S |
| Citation(s) |
24776842 |
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| Submission date |
Mar 11, 2014 |
| Last update date |
Oct 23, 2019 |
| Contact name |
David George Monroe |
| E-mail(s) |
Monroe.David@mayo.edu
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| Phone |
507-538-6517
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| Organization name |
Mayo Clinic
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| Department |
Endocrinology
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| Street address |
200 1st St SW
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| City |
Rochester |
| State/province |
MN |
| ZIP/Postal code |
55905 |
| Country |
USA |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (18)
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| GSM1345812 |
Ad-NERKI, 10 nM E2, rep 1 |
| GSM1345813 |
Ad-NOER, Vehicle, rep 1 |
| GSM1345814 |
Ad-NOER, 10 nM E2, rep 1 |
| GSM1345815 |
Ad-Era, Vehicle, rep 2 |
| GSM1345816 |
Ad-Era, 10 nM E2, rep 2 |
| GSM1345817 |
Ad-NERKI, Vehicle, rep 2 |
| GSM1345818 |
Ad-NERKI, 10 nM E2, rep 2 |
| GSM1345819 |
Ad-NOER, Vehicle, rep 2 |
| GSM1345820 |
Ad-NOER, 10 nM E2, rep 2 |
| GSM1345821 |
Ad-Era, Vehicle, rep 3 |
| GSM1345822 |
Ad-Era, 10 nM E2, rep 3 |
| GSM1345823 |
Ad-NERKI, Vehicle, rep 3 |
| GSM1345824 |
Ad-NERKI, 10 nM E2, rep 3 |
| GSM1345825 |
Ad-NOER, Vehicle, rep 3 |
| GSM1345826 |
Ad-NOER, 10 nM E2, rep 3 |
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| Relations |
| BioProject |
PRJNA240965 |
| SRA |
SRP039909 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE55769_GeneCount.tsv.gz |
3.9 Mb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Processed data are available on Series record |
| Raw data are available in SRA |
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