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Series GSE22643 Query DataSets for GSE22643
Status Public on Jul 01, 2010
Title Expression data from stage 54 Xenopus laevis larvae treated with 20 nM triiodothyronine (T3)
Organism Xenopus laevis
Experiment type Expression profiling by array
Summary Induction of Xenopus laevis larvae metamorphosis is dependent on exposure to TH. Metamorphosis involves the regression, growth or remodeling of almost all the tissues in the animals body.
Metamorphosis in frogs is induced by thyroid hormone. Each organ system has a unique morphological and genetic program that it follows while undergoing metamorphosis involving both the upregulation and downregulation of genes. In this array we examined the change in gene expression in the tail of larvae undergoing precocious metamorphosis following induction with thyroid hormone.
Overall design Whole stage 54 xenopus larvae were exposed to either vehicle for 48 hours or 20 nM T3 for 6 or 48 hours. Total RNA was then purified from tail tissue and the samples were examined by hybridization to the Affymetrix Xenopus array. Developmental stage 51-54 Xenopus larvae were selected because these stages do not express high levels of endogenous T3 but are still capable of morphologically responding to exposure.
Contributor(s) Searcy BT, Propper CR, Stafford P, Beckstrom-Sternberg S, Beckstrom-Sternberg JS, Schwendiman A, Soto-Pena J
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Submission date Jun 30, 2010
Last update date Mar 22, 2012
Contact name Brian Thomas Searcy
Organization name Northern Arizona University
Department Dept of Biological Sciences
Street address P.O. Box 5640, Northern Arizona University
City Flagstaff
State/province AZ
ZIP/Postal code 86011-5640
Country USA
Platforms (1)
GPL1318 [Xenopus_laevis] Affymetrix Xenopus laevis Genome Array
Samples (9)
GSM561846 XenopusTailVehicle-rep1
GSM561847 XenopusTailVehicle-rep2
GSM561848 XenopusTailVehicle-rep3
BioProject PRJNA128041

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE22643_RAW.tar 18.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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