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Sample GSM561854 Query DataSets for GSM561854
Status Public on Jul 01, 2010
Title XenopusTail-T3-48h-rep3
Sample type RNA
Source name Xenopus laevis tail total RNA, stage 51-54 animals, whole animal exposed to 48h T3
Organism Xenopus laevis
Characteristics tissue: tail
stage: stage 54 tadpoles (larvae)
agent: T3
time: 48h
Treatment protocol At time zero vehicle or 20 nM T3 suspended in vehicle was added to the water, tails were collected at 48 h (vehicle), 6 h (T3) and 48 h (T3). Tails were removed, and RNA extracted from pools of three tails/array. Three arrays of pooled tails from each treatment were hybridized.
Growth protocol Stage 54 Xenopus laevis larvae were maintained in 500 ml glass containers at 20C in FETAX. 2/container.
Extracted molecule total RNA
Extraction protocol mRNA was extracted using Qiagen mini-kit RNAeasy protocol according to manufacturer's instructions
Label biotin
Label protocol Samples were labeled using the unmodified Affymetrix labeling protocol
Hybridization protocol Standard Affymetrix Xenopus protocol was used according to manufacturer's instructions
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Description 48h-Rep3
Data processing The data was pre-processed/normalized using the GCRMA algorithm
Submission date Jun 30, 2010
Last update date Jul 01, 2010
Contact name Brian Thomas Searcy
Organization name Northern Arizona University
Department Dept of Biological Sciences
Street address P.O. Box 5640, Northern Arizona University
City Flagstaff
State/province AZ
ZIP/Postal code 86011-5640
Country USA
Platform ID GPL1318
Series (1)
GSE22643 Expression data from stage 54 Xenopus laevis larvae treated with 20 nM triiodothyronine (T3)

Data table header descriptions
VALUE GC-RMA signal intensity

Data table
Xl.8370.2.A1_at 6.047018528
Xl.2422.1.S1_at 8.996378899
Xl.24397.1.S1_at 1.521272421
Xl.7993.1.A1_at 3.361614704
Xl.2921.1.S1_at 2.547132015
Xl.17394.1.S1_at 0.913888752
Xl.9653.1.S1_at 570.6521606
Xl.25524.1.S1_at 3.06140399
Xl.14819.1.A1_at 0.971111655
Xl.14365.1.A1_at 8.308112144
Xl.11042.1.A1_at 0.37568891
Xl.355.2.S1_a_at 89.9092865
Xl.11580.1.S1_at 2.990338326
Xl.6185.1.A1_at 1.399204254
Xl.19342.1.S1_at 2.898166656
Xl.16681.1.A1_at 15.14703655
Xl.408.1.S1_at 2.942882538
Xl.7328.1.A1_at 23.04736328
Xl.24625.1.A1_at 0.254055619
Xl.5960.1.A1_at 2.523540974

Total number of rows: 15611

Table truncated, full table size 435 Kbytes.

Supplementary file Size Download File type/resource
GSM561854.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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