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Sample GSM561847 Query DataSets for GSM561847
Status Public on Jul 01, 2010
Title XenopusTailVehicle-rep2
Sample type RNA
Source name Xenopus laevis tail total RNA, stage 51-54 animals, whole animal exposed to 48h vehicle
Organism Xenopus laevis
Characteristics tissue: tail
stage: stage 54 tadpoles (larvae)
agent: vehicle
time: 48h
Treatment protocol At time zero vehicle or 20 nM T3 suspended in vehicle was added to the water, tails were collected at 48 h (vehicle), 6 h (T3) and 48 h (T3). Tails were removed, and RNA extracted from pools of three tails/array. Three arrays of pooled tails from each treatment were hybridized.
Growth protocol Stage 54 Xenopus laevis larvae were maintained in 500 ml glass containers at 20C in FETAX. 2/container.
Extracted molecule total RNA
Extraction protocol mRNA was extracted using Qiagen mini-kit RNAeasy protocol according to manufacturer's instructions
Label biotin
Label protocol Samples were labeled using the unmodified Affymetrix labeling protocol
Hybridization protocol Standard Affymetrix Xenopus protocol was used according to manufacturer's instructions
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Description Conrol-Rep2
Data processing The data was pre-processed/normalized using the GCRMA algorithm
Submission date Jun 30, 2010
Last update date Jul 01, 2010
Contact name Brian Thomas Searcy
Organization name Northern Arizona University
Department Dept of Biological Sciences
Street address P.O. Box 5640, Northern Arizona University
City Flagstaff
State/province AZ
ZIP/Postal code 86011-5640
Country USA
Platform ID GPL1318
Series (1)
GSE22643 Expression data from stage 54 Xenopus laevis larvae treated with 20 nM triiodothyronine (T3)

Data table header descriptions
VALUE GC-RMA signal intensity

Data table
Xl.8370.2.A1_at 5.410229683
Xl.2422.1.S1_at 5.198698997
Xl.24397.1.S1_at 1.592247486
Xl.7993.1.A1_at 3.324724674
Xl.2921.1.S1_at 2.548036814
Xl.17394.1.S1_at 0.990298212
Xl.9653.1.S1_at 636.0122681
Xl.25524.1.S1_at 4.68237114
Xl.14819.1.A1_at 0.847345054
Xl.14365.1.A1_at 2.133999586
Xl.11042.1.A1_at 0.407885492
Xl.355.2.S1_a_at 11.72590065
Xl.11580.1.S1_at 2.252149105
Xl.6185.1.A1_at 1.008790493
Xl.19342.1.S1_at 1.579247236
Xl.16681.1.A1_at 15.58000469
Xl.408.1.S1_at 9.966096878
Xl.7328.1.A1_at 28.17293549
Xl.24625.1.A1_at 0.661860645
Xl.5960.1.A1_at 1.58187449

Total number of rows: 15611

Table truncated, full table size 435 Kbytes.

Supplementary file Size Download File type/resource
GSM561847.CEL.gz 2.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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