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Sample GSM561848 Query DataSets for GSM561848
Status Public on Jul 01, 2010
Title XenopusTailVehicle-rep3
Sample type RNA
Source name Xenopus laevis tail total RNA, stage 51-54 animals, whole animal exposed to 48h vehicle
Organism Xenopus laevis
Characteristics tissue: tail
stage: stage 54 tadpoles (larvae)
agent: vehicle
time: 48h
Treatment protocol At time zero vehicle or 20 nM T3 suspended in vehicle was added to the water, tails were collected at 48 h (vehicle), 6 h (T3) and 48 h (T3). Tails were removed, and RNA extracted from pools of three tails/array. Three arrays of pooled tails from each treatment were hybridized.
Growth protocol Stage 54 Xenopus laevis larvae were maintained in 500 ml glass containers at 20C in FETAX. 2/container.
Extracted molecule total RNA
Extraction protocol mRNA was extracted using Qiagen mini-kit RNAeasy protocol according to manufacturer's instructions
Label biotin
Label protocol Samples were labeled using the unmodified Affymetrix labeling protocol
Hybridization protocol Standard Affymetrix Xenopus protocol was used according to manufacturer's instructions
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Description Control-Rep3
Data processing The data was pre-processed/normalized using the GCRMA algorithm
Submission date Jun 30, 2010
Last update date Jul 01, 2010
Contact name Brian Thomas Searcy
Organization name Northern Arizona University
Department Dept of Biological Sciences
Street address P.O. Box 5640, Northern Arizona University
City Flagstaff
State/province AZ
ZIP/Postal code 86011-5640
Country USA
Platform ID GPL1318
Series (1)
GSE22643 Expression data from stage 54 Xenopus laevis larvae treated with 20 nM triiodothyronine (T3)

Data table header descriptions
VALUE GC-RMA signal intensity

Data table
Xl.8370.2.A1_at 5.327083588
Xl.2422.1.S1_at 5.950918198
Xl.24397.1.S1_at 1.672712088
Xl.7993.1.A1_at 3.384260178
Xl.2921.1.S1_at 2.52236557
Xl.17394.1.S1_at 1.019261003
Xl.9653.1.S1_at 626.7513428
Xl.25524.1.S1_at 4.641568184
Xl.14819.1.A1_at 0.842832446
Xl.14365.1.A1_at 1.953395367
Xl.11042.1.A1_at 0.438554943
Xl.355.2.S1_a_at 14.10743999
Xl.11580.1.S1_at 2.301282644
Xl.6185.1.A1_at 0.925975382
Xl.19342.1.S1_at 1.428746819
Xl.16681.1.A1_at 15.21667099
Xl.408.1.S1_at 9.100429535
Xl.7328.1.A1_at 26.60815239
Xl.24625.1.A1_at 0.667235136
Xl.5960.1.A1_at 1.631316066

Total number of rows: 15611

Table truncated, full table size 435 Kbytes.

Supplementary file Size Download File type/resource
GSM561848.CEL.gz 2.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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