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Sample GSM561850 Query DataSets for GSM561850
Status Public on Jul 01, 2010
Title XenopusTail-T3-6h-rep2
Sample type RNA
 
Source name Xenopus laevis tail total RNA, stage 51-54 animals, whole animal exposed to 6h T3
Organism Xenopus laevis
Characteristics tissue: tail
stage: stage 54 tadpoles (larvae)
agent: T3
time: 6h
Treatment protocol At time zero vehicle or 20 nM T3 suspended in vehicle was added to the water, tails were collected at 48 h (vehicle), 6 h (T3) and 48 h (T3). Tails were removed, and RNA extracted from pools of three tails/array. Three arrays of pooled tails from each treatment were hybridized.
Growth protocol Stage 54 Xenopus laevis larvae were maintained in 500 ml glass containers at 20C in FETAX. 2/container.
Extracted molecule total RNA
Extraction protocol mRNA was extracted using Qiagen mini-kit RNAeasy protocol according to manufacturer's instructions
Label biotin
Label protocol Samples were labeled using the unmodified Affymetrix labeling protocol
 
Hybridization protocol Standard Affymetrix Xenopus protocol was used according to manufacturer's instructions
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Description 6h-Rep2
Data processing The data was pre-processed/normalized using the GCRMA algorithm
 
Submission date Jun 30, 2010
Last update date Jul 01, 2010
Contact name Brian Thomas Searcy
E-mail(s) btsearcy@gmail.com
Organization name Northern Arizona University
Department Dept of Biological Sciences
Street address P.O. Box 5640, Northern Arizona University
City Flagstaff
State/province AZ
ZIP/Postal code 86011-5640
Country USA
 
Platform ID GPL1318
Series (1)
GSE22643 Expression data from stage 54 Xenopus laevis larvae treated with 20 nM triiodothyronine (T3)

Data table header descriptions
ID_REF
VALUE GC-RMA signal intensity

Data table
ID_REF VALUE
Xl.8370.2.A1_at 8.622058868
Xl.2422.1.S1_at 18.94011307
Xl.24397.1.S1_at 2.287756681
Xl.7993.1.A1_at 3.777728081
Xl.2921.1.S1_at 1.899067521
Xl.17394.1.S1_at 0.767460585
Xl.9653.1.S1_at 694.4467163
Xl.25524.1.S1_at 3.001291513
Xl.14819.1.A1_at 0.598560393
Xl.14365.1.A1_at 4.922479153
Xl.11042.1.A1_at 0.239843905
Xl.355.2.S1_a_at 22.73995209
Xl.11580.1.S1_at 2.757895708
Xl.6185.1.A1_at 1.996407509
Xl.19342.1.S1_at 2.284817457
Xl.16681.1.A1_at 19.1523838
Xl.408.1.S1_at 5.141138554
Xl.7328.1.A1_at 34.17993927
Xl.24625.1.A1_at 1.414320827
Xl.5960.1.A1_at 0.964592457

Total number of rows: 15611

Table truncated, full table size 435 Kbytes.




Supplementary file Size Download File type/resource
GSM561850.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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