|
| Status |
Public on Jul 01, 2010 |
| Title |
XenopusTail-T3-6h-rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Xenopus laevis tail total RNA, stage 51-54 animals, whole animal exposed to 6h T3
|
| Organism |
Xenopus laevis |
| Characteristics |
tissue: tail
stage: stage 54 tadpoles (larvae)
agent: T3
time: 6h
|
| Treatment protocol |
At time zero vehicle or 20 nM T3 suspended in vehicle was added to the water, tails were collected at 48 h (vehicle), 6 h (T3) and 48 h (T3). Tails were removed, and RNA extracted from pools of three tails/array. Three arrays of pooled tails from each treatment were hybridized.
|
| Growth protocol |
Stage 54 Xenopus laevis larvae were maintained in 500 ml glass containers at 20C in FETAX. 2/container.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
mRNA was extracted using Qiagen mini-kit RNAeasy protocol according to manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Samples were labeled using the unmodified Affymetrix labeling protocol
|
| |
|
| Hybridization protocol |
Standard Affymetrix Xenopus protocol was used according to manufacturer's instructions
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
6h-Rep3
|
| Data processing |
The data was pre-processed/normalized using the GCRMA algorithm
|
| |
|
| Submission date |
Jun 30, 2010 |
| Last update date |
Jul 01, 2010 |
| Contact name |
Brian Thomas Searcy |
| E-mail(s) |
btsearcy@gmail.com
|
| Organization name |
Northern Arizona University
|
| Department |
Dept of Biological Sciences
|
| Street address |
P.O. Box 5640, Northern Arizona University
|
| City |
Flagstaff |
| State/province |
AZ |
| ZIP/Postal code |
86011-5640 |
| Country |
USA |
| |
|
| Platform ID |
GPL1318 |
| Series (1) |
| GSE22643 |
Expression data from stage 54 Xenopus laevis larvae treated with 20 nM triiodothyronine (T3) |
|
