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Sample GSM561851 Query DataSets for GSM561851
Status Public on Jul 01, 2010
Title XenopusTail-T3-6h-rep3
Sample type RNA
Source name Xenopus laevis tail total RNA, stage 51-54 animals, whole animal exposed to 6h T3
Organism Xenopus laevis
Characteristics tissue: tail
stage: stage 54 tadpoles (larvae)
agent: T3
time: 6h
Treatment protocol At time zero vehicle or 20 nM T3 suspended in vehicle was added to the water, tails were collected at 48 h (vehicle), 6 h (T3) and 48 h (T3). Tails were removed, and RNA extracted from pools of three tails/array. Three arrays of pooled tails from each treatment were hybridized.
Growth protocol Stage 54 Xenopus laevis larvae were maintained in 500 ml glass containers at 20C in FETAX. 2/container.
Extracted molecule total RNA
Extraction protocol mRNA was extracted using Qiagen mini-kit RNAeasy protocol according to manufacturer's instructions
Label biotin
Label protocol Samples were labeled using the unmodified Affymetrix labeling protocol
Hybridization protocol Standard Affymetrix Xenopus protocol was used according to manufacturer's instructions
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Description 6h-Rep3
Data processing The data was pre-processed/normalized using the GCRMA algorithm
Submission date Jun 30, 2010
Last update date Jul 01, 2010
Contact name Brian Thomas Searcy
Organization name Northern Arizona University
Department Dept of Biological Sciences
Street address P.O. Box 5640, Northern Arizona University
City Flagstaff
State/province AZ
ZIP/Postal code 86011-5640
Country USA
Platform ID GPL1318
Series (1)
GSE22643 Expression data from stage 54 Xenopus laevis larvae treated with 20 nM triiodothyronine (T3)

Data table header descriptions
VALUE GC-RMA signal intensity

Data table
Xl.8370.2.A1_at 8.503294945
Xl.2422.1.S1_at 18.4153862
Xl.24397.1.S1_at 2.346177578
Xl.7993.1.A1_at 3.749876738
Xl.2921.1.S1_at 1.956774712
Xl.17394.1.S1_at 0.783317685
Xl.9653.1.S1_at 705.192749
Xl.25524.1.S1_at 2.660364628
Xl.14819.1.A1_at 0.607579947
Xl.14365.1.A1_at 4.349421024
Xl.11042.1.A1_at 0.237012386
Xl.355.2.S1_a_at 21.91484642
Xl.11580.1.S1_at 2.892649651
Xl.6185.1.A1_at 1.894276261
Xl.19342.1.S1_at 2.319760561
Xl.16681.1.A1_at 19.34602165
Xl.408.1.S1_at 5.177204132
Xl.7328.1.A1_at 35.61170197
Xl.24625.1.A1_at 1.626623273
Xl.5960.1.A1_at 1.030022383

Total number of rows: 15611

Table truncated, full table size 435 Kbytes.

Supplementary file Size Download File type/resource
GSM561851.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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