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Sample GSM561846 Query DataSets for GSM561846
Status Public on Jul 01, 2010
Title XenopusTailVehicle-rep1
Sample type RNA
 
Source name Xenopus laevis tail total RNA, stage 51-54 animals, whole animal exposed to 48h vehicle
Organism Xenopus laevis
Characteristics tissue: tail
stage: stage 54 tadpoles (larvae)
agent: vehicle
time: 48h
Treatment protocol At time zero vehicle or 20 nM T3 suspended in vehicle was added to the water, tails were collected at 48 h (vehicle), 6 h (T3) and 48 h (T3). Tails were removed, and RNA extracted from pools of three tails/array. Three arrays of pooled tails from each treatment were hybridized.
Growth protocol Stage 54 Xenopus laevis larvae were maintained in 500 ml glass containers at 20C in FETAX. 2/container.
Extracted molecule total RNA
Extraction protocol mRNA was extracted using Qiagen mini-kit RNAeasy protocol according to manufacturer's instructions
Label biotin
Label protocol Samples were labeled using the unmodified Affymetrix labeling protocol
 
Hybridization protocol Standard Affymetrix Xenopus protocol was used according to manufacturer's instructions
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Description Control-Rep1
Data processing The data was pre-processed/normalized using the GCRMA algorithm
 
Submission date Jun 30, 2010
Last update date Jul 01, 2010
Contact name Brian Thomas Searcy
E-mail(s) btsearcy@gmail.com
Organization name Northern Arizona University
Department Dept of Biological Sciences
Street address P.O. Box 5640, Northern Arizona University
City Flagstaff
State/province AZ
ZIP/Postal code 86011-5640
Country USA
 
Platform ID GPL1318
Series (1)
GSE22643 Expression data from stage 54 Xenopus laevis larvae treated with 20 nM triiodothyronine (T3)

Data table header descriptions
ID_REF
VALUE GC-RMA signal intensity

Data table
ID_REF VALUE
Xl.8370.2.A1_at 5.457304478
Xl.2422.1.S1_at 4.820803165
Xl.24397.1.S1_at 1.670712352
Xl.7993.1.A1_at 3.353356123
Xl.2921.1.S1_at 2.516671181
Xl.17394.1.S1_at 0.983759403
Xl.9653.1.S1_at 632.3449097
Xl.25524.1.S1_at 4.500196457
Xl.14819.1.A1_at 0.804923594
Xl.14365.1.A1_at 2.083908081
Xl.11042.1.A1_at 0.42084828
Xl.355.2.S1_a_at 12.22845745
Xl.11580.1.S1_at 2.271544695
Xl.6185.1.A1_at 1.100132942
Xl.19342.1.S1_at 1.346732855
Xl.16681.1.A1_at 14.44046211
Xl.408.1.S1_at 10.39852619
Xl.7328.1.A1_at 26.84384155
Xl.24625.1.A1_at 0.574954033
Xl.5960.1.A1_at 1.709810257

Total number of rows: 15611

Table truncated, full table size 435 Kbytes.




Supplementary file Size Download File type/resource
GSM561846.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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