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Sample GSM561853 Query DataSets for GSM561853
Status Public on Jul 01, 2010
Title XenopusTail-T3-48h-rep2
Sample type RNA
Source name Xenopus laevis tail total RNA, stage 51-54 animals, whole animal exposed to 48h T3
Organism Xenopus laevis
Characteristics tissue: tail
stage: stage 54 tadpoles (larvae)
agent: T3
time: 48h
Treatment protocol At time zero vehicle or 20 nM T3 suspended in vehicle was added to the water, tails were collected at 48 h (vehicle), 6 h (T3) and 48 h (T3). Tails were removed, and RNA extracted from pools of three tails/array. Three arrays of pooled tails from each treatment were hybridized.
Growth protocol Stage 54 Xenopus laevis larvae were maintained in 500 ml glass containers at 20C in FETAX. 2/container.
Extracted molecule total RNA
Extraction protocol mRNA was extracted using Qiagen mini-kit RNAeasy protocol according to manufacturer's instructions
Label biotin
Label protocol Samples were labeled using the unmodified Affymetrix labeling protocol
Hybridization protocol Standard Affymetrix Xenopus protocol was used according to manufacturer's instructions
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Description 48h-Rep2
Data processing The data was pre-processed/normalized using the GCRMA algorithm
Submission date Jun 30, 2010
Last update date Jul 01, 2010
Contact name Brian Thomas Searcy
Organization name Northern Arizona University
Department Dept of Biological Sciences
Street address P.O. Box 5640, Northern Arizona University
City Flagstaff
State/province AZ
ZIP/Postal code 86011-5640
Country USA
Platform ID GPL1318
Series (1)
GSE22643 Expression data from stage 54 Xenopus laevis larvae treated with 20 nM triiodothyronine (T3)

Data table header descriptions
VALUE GC-RMA signal intensity

Data table
Xl.8370.2.A1_at 6.105038166
Xl.2422.1.S1_at 10.67563248
Xl.24397.1.S1_at 1.332338572
Xl.7993.1.A1_at 3.371236324
Xl.2921.1.S1_at 2.074786663
Xl.17394.1.S1_at 1.266394138
Xl.9653.1.S1_at 692.2036743
Xl.25524.1.S1_at 3.456248522
Xl.14819.1.A1_at 0.73520261
Xl.14365.1.A1_at 10.24028683
Xl.11042.1.A1_at 0.362779975
Xl.355.2.S1_a_at 100.0118713
Xl.11580.1.S1_at 3.366307974
Xl.6185.1.A1_at 1.08929038
Xl.19342.1.S1_at 3.305622339
Xl.16681.1.A1_at 17.11579132
Xl.408.1.S1_at 2.667919397
Xl.7328.1.A1_at 31.04070282
Xl.24625.1.A1_at 0.229462609
Xl.5960.1.A1_at 3.323987484

Total number of rows: 15611

Table truncated, full table size 435 Kbytes.

Supplementary file Size Download File type/resource
GSM561853.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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