GEO DataSets

(Submitter supplied) A genetically diverse strain (labelled as London) of the phytophagous mite Tetranychus urticae was transferred from its common host (bean) to other host plants (cotton, maize or soy). Three different host plant species were included in the experimental set-up: cotton (Gossypium spp.), maize (Zea mays cv. Ronaldinio) and soy (Glycine max cv. Merlin). Five generations after host-transfer, total RNA of all mite populations (London, London-SOY, London-MAIZE and London-COTTON) was collected and used in a genome-wide gene expression microarray (Sureprint G3 microarray, Agilent) Microarray analysis revealed large-scale differential expression of genes coding for enzymes of detoxification families, secreted proteins with unknown functions and regulatory enzymes.

Organism:

Tetranychus urticae

Type:

Expression profiling by array

Platform:

GPL16890

11 Samples

Download data: TXT

(Submitter supplied) Proteins produced in the salivary glands of arthropod herbivores can function as effectors to modify plant defense responses. To obtain a set of candidate, salivary gland specific genes for the mite herbivore Tetranychus urticae, mites were dissected and the head region was recovered. Specifically, the proterosoma was dissected from 250 mites. This includes the salivary glands, and other nearby tissue (given their small size, exact micro-dissection of salivary glands was not possible). more...

Organism:

Tetranychus urticae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21813

4 Samples

Download data: TXT

(Submitter supplied) In an experimental evolutionary set-up, we transferred a genetically diverse strain of the spider mite Tetranychus urticae from its common host bean to tomato where replicated populations were allowed to adapt. By sampling the transcriptomes of non-adapted and adaptes mites feeding on bean and tomato, we identified gene-expression changes in the spider mite affiliated with tomato adaptation. Transcriptional analysis revealed that both constitutive gene-expression levels as well as the transcriptional plasticity of genes were affected. more...

Organism:

Tetranychus urticae

Type:

Expression profiling by array

Platform:

GPL16890

12 Samples

Download data: TXT

(Submitter supplied) 250 adult T. urticae females from the London strain (grown on acyanogenic P. vulgaris cv. Prelude bean plants) were transferred to cyanogenic P. lunatus cv. 8078 bean plants. Thirty-five generations after the host transfer, total RNA was extracted from mites growing on both bean species (London and London-CYANO strain) and used in in a genome-wide gene expression microarray (Sureprint G3 microarray, Agilent) experiment to assess significantly differentially expressed genes (FC ≥ 2 and FDR-corrected p-value < 0.05) between mites grown on P. more...

Organism:

Tetranychus urticae

Type:

Expression profiling by array

Platform:

GPL16890

4 Samples

Download data: TXT

(Submitter supplied) The goal of our microarray experiments was twofold: 1) Compare the gene expression profile of acaricide resistant spider mite strains (MAR-AB and MR-VP) with that of a susceptible spider mite strain (London); 2) Study gene expression changes in spider mites from the London strain upon transfer from bean, a suitable host, to tomato, a less favorable host. These gene expression changes upon host change were measured for three timepoints (2 hour on tomato (Tomato-2h), 12 hour on tomato (Tomato-12h) and 5 generations on tomato (Tomato-5G)).

Organism:

Tetranychus urticae

Type:

Expression profiling by array

Platform:

GPL15756

23 Samples

Download data: TXT

(Submitter supplied) Tomato plants are commonly attacked by herbivorous mites, including by generalist Tetranychus urticae and specialists Tetranychus evansi and Aculops lycopersici. Mite feeding induces plant defense responses that reduce mite performance. However, via poorly understood mechanisms, T. evansi and A. lycopersici suppress plant defenses and, consequently, maintain a high performance on tomato. Accordingly, on a shared host, non-adapted T. more...

Organism:

Solanum lycopersicum

Type:

Expression profiling by array

Platform:

GPL25309

24 Samples

Download data: FTR

(Submitter supplied) Investigating essential physiological processes in diapausing mites by analyzing genome wide gene expression changes using custom-built microarray. We investigated the molecular biology of facultative reproductive diapause in the chelicerate Tetranychus urticae (Acari: Tetranychidae) by analyzing genome-wide gene expression differences in diapausing and non diapausing T. urticae, using an Agilent custom-built two color gene expression microarray. more...

Organism:

Tetranychus urticae

Type:

Expression profiling by array

Platform:

GPL16890

4 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Tetranychus urticae

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

Platform:

GPL14176

26 Samples

Download data: TXT

(Submitter supplied) We generated 38-bp Illumina reads from messenger RNA libraries from mites transferred from their preferred laboratory host, bean (Phaseolus vulgaris cv. California Red Kidney), to one of three hosts: bean, Arabidopsis thaliana (Bla-2 accession) and the tomato (Solanum lycopersicum; genotype Heinz 1706). Larvae were carefully collected from bean plants and transferred to the treatment plant. Mites were reared on these plants for ~24 hours, after which mites were collected for mRNA library preparation. more...

Organism:

Tetranychus urticae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL14176

9 Samples

Download data: TXT

(Submitter supplied) We generated 38-bp Illumina reads from single messenger RNA libraries from three diverse developmental stages of the two-spotted spider mite to capture small RNA diversity across development. Adult, nymphal+larvae and embryonic stages were separated using sieves of various pore sizes, and mites of various developmental stages were carefully selected for small RNA library preparation. Samples were a mix of males and females to capture male and female patterns of small RNA composition and were reared on beans (Phaseolus vulgaris cv California Red Kidney). more...

Organism:

Tetranychus urticae

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL14176

3 Samples

Download data: TXT

(Submitter supplied) We generated 77-bp Illumina reads from single messenger RNA libraries from four diverse developmental stages of the two-spotted spider mite to maximally capture the complement of transcribed sequences across development. Adult, nymphal, larvae and embryonic stages were separated using sieves of various pore sizes, and mites of various developmental stages were carefully selected for transcriptome library preparation. more...

Organism:

Tetranychus urticae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL14176

8 Samples

Download data: TXT

(Submitter supplied) We sequenced messenger RNA from mixed stages of the two-spotted spider mite (Tetranychus urticae) reared on bean (Phaseolus vulgaris cv California Red Kidney; the laboratory host plant for mites) and two Arabidopsis thaliana accessions which were considered to either be susceptible (Kondara) or resistant (Bla-2) to mite feeding. This pilot experiment was conducted to assess gene expression differences of mites grown on sensitive versus resistant Arabidopsis accessions, as well as differences in mites feeding on different host species. more...

Organism:

Tetranychus urticae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL14176

6 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Solanum lycopersicum

Type:

Expression profiling by array

Platform:

GPL16358

33 Samples

Download data: CEL

(Submitter supplied) The two-spotted spider mite, Tetranychus urticae, is one of the most significant mite pests in agriculture that can feed on more than 1,100 plant hosts, including model plants Arabidopsis thaliana and tomato, Solanum lycopersicum. In order to refine the involvement of jasmonic acid (JA) in mite-induced responses, we analyzed transcriptional changes in tomato JA signaling mutant defenseless1 (def-1) upon JA treatment and spider mite herbivory. more...

Organism:

Solanum lycopersicum

Type:

Expression profiling by array

Platform:

GPL16358

12 Samples

Download data: CEL, TXT

(Submitter supplied) The two-spotted spider mite, Tetranychus urticae, is one of the most significant mite pests in agriculture that can feed on more than 1,100 plant hosts, including model plants Arabidopsis thaliana and tomato, Solanum lycopersicum. Here, we described tomato transcriptional responses to spider mite feeding and compared them to Arabidopsis in order to determine conserved and divergent responses to this pest. more...

Organism:

Solanum lycopersicum

Type:

Expression profiling by array

Platform:

GPL16358

21 Samples

Download data: CEL, TXT

(Submitter supplied) While pathogen-induced immunity is comparatively well characterized, far less is known about plant defense responses to arthropod herbivores. To date, most molecular-genetic studies of plant-arthropod interactions have focused on insects. However, plant-feeding (phytophagous) mites are also pests of diverse plants, and mites induce different patterns of damage to plant tissues than do well-studied insects (e.g., Lepidopteran larvae or aphids). more...

Organism:

Arabidopsis thaliana

Type:

Expression profiling by array

Platform:

GPL16112

28 Samples

Download data: CEL

(Submitter supplied) Our goal was to investigate the transcriptional response of T. urticae to different Arabidopsis plants with varying levels of indole glucosinolates.

Organism:

Tetranychus urticae

Type:

Expression profiling by array

Platform:

GPL16890

12 Samples

Download data: TXT

(Submitter supplied) Generalist arthropod herbivores rapidly adapt to a broad range of host plants. However, the extent of transcriptional reprogramming in the herbivore and its hosts associated with adaptation remains poorly understood. Using the spider mite Tetranychus urticae and tomato as models with available genomic resources, we investigated the reciprocal genome-wide transcriptional changes in both spider mite and tomato as a consequence of mite’s adaptation to tomato We used microarray to assess global gene expression in Solanum lycopersicum cv. more...

Organism:

Solanum lycopersicum

Type:

Expression profiling by array

Platform:

GPL16358

20 Samples

Download data: CEL

(Submitter supplied) The red spider mite, Tetranychus evansi, is a oligophagous specialist mite pest of Solanaceae plants. Here, we described tomato transcriptional responses to T. evansi feeding and compared them to responses to tomato-adapted and -non-adapted strains of generalist herbivorous spider mite Tetranychus urticae. We used microarray to assess global gene expression in Solanum lycopersicum cv. Heinz 1706 upon T. more...

Organism:

Solanum lycopersicum

Type:

Expression profiling by array

Platform:

GPL16358

6 Samples

Download data: CEL

(Submitter supplied) Spider mites, including the two-spotted spider mite (Tetranychus urticae, TSSM) and the Banks grass mite (Oligonychus pratensis, BGM), are becoming increasingly important agricultural pests. The TSSM is an extreme generalist documented to feed on more than 1100 plant hosts. In contrast, the BGM is a grass specialist, with hosts including important cereal crops like maize, wheat, and sorghum. Historically, studies of plant-herbivore interactions have focused largely on insects. more...

Organism:

Zea mays

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17628

28 Samples

Download data: TXT