NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE68708 Query DataSets for GSE68708
Status Public on Jul 21, 2015
Title Host plant adaptation of a polyphagous herbivore shapes transcriptome of both herbivore and host
Organism Tetranychus urticae
Experiment type Expression profiling by array
Summary In an experimental evolutionary set-up, we transferred a genetically diverse strain of the spider mite Tetranychus urticae from its common host bean to tomato where replicated populations were allowed to adapt.
By sampling the transcriptomes of non-adapted and adaptes mites feeding on bean and tomato, we identified gene-expression changes in the spider mite affiliated with tomato adaptation.
Transcriptional analysis revealed that both constitutive gene-expression levels as well as the transcriptional plasticity of genes were affected.
Specifically, tomato adaptation resulted in a large set of constitutively down-regulated genes of unknown function in adapted mites compared to non-adapted mites. Additionally, upon tomato exposure, adapted mites exhibited an increased transcriptional plasticity of genes coding for detoxifying enzymes and xenobiotic transporters. Remarkably, adapted mites further exhibited a differential effect on host plant physiology compared to non-adapted mites. Adapted mites induced a greater chlorotic area on tomato leaves and triggered attenuated induced responses relative to those induced by non-adapted mites.
 
Overall design 12 comparisons were investigated; 3 biological replicates for 4 conditions: (1) NonAdapted-Induced (non-adapted 24 hrs on tomato vs non-adapted on bean); (2) Adapted-Tomato (adapted on tomato vs non-adapted on bean); (3) Adapted-2G Bean (adapted 2 generations on bean vs non-adapted on bean); (4) Adapted-2G Bean-Induced (adapted 2 generations on bean, 24 hrs on tomato vs non-adapted on bean).
 
Contributor(s) Wybouw N, Zhurov V, Grbic V, Van Leeuwen T
Citation(s) 26211543, 30333191
Submission date May 09, 2015
Last update date Dec 26, 2018
Contact name Nicky Wybouw
Organization name University of Ghent
Department Department of Crop Protection
Street address Coupure Links 653
City Gent
ZIP/Postal code 9000
Country Belgium
 
Platforms (1)
GPL16890 Agilent-033850 Tetranychus urticae 63K array
Samples (12)
GSM1679380 NonAdapted-Induced1
GSM1679381 NonAdapted-Induced2
GSM1679382 NonAdapted-Induced3
Relations
BioProject PRJNA283452

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE68708_RAW.tar 72.2 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap