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| Status |
Public on Jul 21, 2015 |
| Title |
Host plant adaptation of a polyphagous herbivore shapes transcriptome of both herbivore and host |
| Organism |
Tetranychus urticae |
| Experiment type |
Expression profiling by array
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| Summary |
In an experimental evolutionary set-up, we transferred a genetically diverse strain of the spider mite Tetranychus urticae from its common host bean to tomato where replicated populations were allowed to adapt.
By sampling the transcriptomes of non-adapted and adaptes mites feeding on bean and tomato, we identified gene-expression changes in the spider mite affiliated with tomato adaptation.
Transcriptional analysis revealed that both constitutive gene-expression levels as well as the transcriptional plasticity of genes were affected.
Specifically, tomato adaptation resulted in a large set of constitutively down-regulated genes of unknown function in adapted mites compared to non-adapted mites. Additionally, upon tomato exposure, adapted mites exhibited an increased transcriptional plasticity of genes coding for detoxifying enzymes and xenobiotic transporters. Remarkably, adapted mites further exhibited a differential effect on host plant physiology compared to non-adapted mites. Adapted mites induced a greater chlorotic area on tomato leaves and triggered attenuated induced responses relative to those induced by non-adapted mites.
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| Overall design |
12 comparisons were investigated; 3 biological replicates for 4 conditions: (1) NonAdapted-Induced (non-adapted 24 hrs on tomato vs non-adapted on bean); (2) Adapted-Tomato (adapted on tomato vs non-adapted on bean); (3) Adapted-2G Bean (adapted 2 generations on bean vs non-adapted on bean); (4) Adapted-2G Bean-Induced (adapted 2 generations on bean, 24 hrs on tomato vs non-adapted on bean).
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| Contributor(s) |
Wybouw N, Zhurov V, Grbic V, Van Leeuwen T |
| Citation(s) |
26211543, 30333191 |
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| Submission date |
May 09, 2015 |
| Last update date |
Dec 26, 2018 |
| Contact name |
Nicky Wybouw |
| Organization name |
University of Ghent
|
| Department |
Department of Crop Protection
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| Street address |
Coupure Links 653
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| City |
Gent |
| ZIP/Postal code |
9000 |
| Country |
Belgium |
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| Platforms (1) |
| GPL16890 |
Agilent-033850 Tetranychus urticae 63K array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA283452 |
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