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Links from GEO DataSets

Items: 19

1.

The genome of Tetranychus urticae reveals herbivorous pest adaptations

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.
Organism:
Tetranychus urticae
Type:
Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing
Platform:
GPL14176
26 Samples
Download data: TXT
Series
Accession:
GSE32342
ID:
200032342
2.

Transcriptional responses of the two-spotted spider mite (Tetranychus urticae) after transfer to different plant hosts

(Submitter supplied) We generated 38-bp Illumina reads from messenger RNA libraries from mites transferred from their preferred laboratory host, bean (Phaseolus vulgaris cv. California Red Kidney), to one of three hosts: bean, Arabidopsis thaliana (Bla-2 accession) and the tomato (Solanum lycopersicum; genotype Heinz 1706). Larvae were carefully collected from bean plants and transferred to the treatment plant. Mites were reared on these plants for ~24 hours, after which mites were collected for mRNA library preparation. more...
Organism:
Tetranychus urticae
Type:
Expression profiling by high throughput sequencing
Platform:
GPL14176
9 Samples
Download data: TXT
Series
Accession:
GSE32009
ID:
200032009
3.

Developmental stage-specific small RNA composition in the two-spotted spider mite (Tetranychus urticae)

(Submitter supplied) We generated 38-bp Illumina reads from single messenger RNA libraries from three diverse developmental stages of the two-spotted spider mite to capture small RNA diversity across development. Adult, nymphal+larvae and embryonic stages were separated using sieves of various pore sizes, and mites of various developmental stages were carefully selected for small RNA library preparation. Samples were a mix of males and females to capture male and female patterns of small RNA composition and were reared on beans (Phaseolus vulgaris cv California Red Kidney). more...
Organism:
Tetranychus urticae
Type:
Non-coding RNA profiling by high throughput sequencing
Platform:
GPL14176
3 Samples
Download data: TXT
Series
Accession:
GSE32005
ID:
200032005
4.

Developmental stage-specific gene expression in the two-spotted spider mite (Tetranychus urticae)

(Submitter supplied) We generated 77-bp Illumina reads from single messenger RNA libraries from four diverse developmental stages of the two-spotted spider mite to maximally capture the complement of transcribed sequences across development. Adult, nymphal, larvae and embryonic stages were separated using sieves of various pore sizes, and mites of various developmental stages were carefully selected for transcriptome library preparation. more...
Organism:
Tetranychus urticae
Type:
Expression profiling by high throughput sequencing
Platform:
GPL14176
8 Samples
Download data: TXT
Series
Accession:
GSE31527
ID:
200031527
5.

Spider mite preliminary feeding experiment with mites reared on bean and two Arabidopsis thaliana accessions

(Submitter supplied) We sequenced messenger RNA from mixed stages of the two-spotted spider mite (Tetranychus urticae) reared on bean (Phaseolus vulgaris cv California Red Kidney; the laboratory host plant for mites) and two Arabidopsis thaliana accessions which were considered to either be susceptible (Kondara) or resistant (Bla-2) to mite feeding. This pilot experiment was conducted to assess gene expression differences of mites grown on sensitive versus resistant Arabidopsis accessions, as well as differences in mites feeding on different host species. more...
Organism:
Tetranychus urticae
Type:
Expression profiling by high throughput sequencing
Platform:
GPL14176
6 Samples
Download data: TXT
Series
Accession:
GSE31525
ID:
200031525
6.

A link between host plant adaptation and pesticide resistance in the polyphagous spider mite Tetranychus urticae

(Submitter supplied) The goal of our microarray experiments was twofold: 1) Compare the gene expression profile of acaricide resistant spider mite strains (MAR-AB and MR-VP) with that of a susceptible spider mite strain (London); 2) Study gene expression changes in spider mites from the London strain upon transfer from bean, a suitable host, to tomato, a less favorable host. These gene expression changes upon host change were measured for three timepoints (2 hour on tomato (Tomato-2h), 12 hour on tomato (Tomato-12h) and 5 generations on tomato (Tomato-5G)).
Organism:
Tetranychus urticae
Type:
Expression profiling by array
Platform:
GPL15756
23 Samples
Download data: TXT
Series
Accession:
GSE39869
ID:
200039869
7.

Gene expression data for body versus head samples for detection of genes with enriched expression in salivary glands of the spider mite Tetranychus urticae

(Submitter supplied) Proteins produced in the salivary glands of arthropod herbivores can function as effectors to modify plant defense responses. To obtain a set of candidate, salivary gland specific genes for the mite herbivore Tetranychus urticae, mites were dissected and the head region was recovered. Specifically, the proterosoma was dissected from 250 mites. This includes the salivary glands, and other nearby tissue (given their small size, exact micro-dissection of salivary glands was not possible). more...
Organism:
Tetranychus urticae
Type:
Expression profiling by high throughput sequencing
Platform:
GPL21813
4 Samples
Download data: TXT
Series
Accession:
GSE81128
ID:
200081128
8.

Genome wide gene-expression analysis of the spider mite Tetranychus urticae after transfer from their common host (bean) to either cotton, maize or soy

(Submitter supplied) A genetically diverse strain (labelled as London) of the phytophagous mite Tetranychus urticae was transferred from its common host (bean) to other host plants (cotton, maize or soy). Three different host plant species were included in the experimental set-up: cotton (Gossypium spp.), maize (Zea mays cv. Ronaldinio) and soy (Glycine max cv. Merlin). Five generations after host-transfer, total RNA of all mite populations (London, London-SOY, London-MAIZE and London-COTTON) was collected and used in a genome-wide gene expression microarray (Sureprint G3 microarray, Agilent) Microarray analysis revealed large-scale differential expression of genes coding for enzymes of detoxification families, secreted proteins with unknown functions and regulatory enzymes.
Organism:
Tetranychus urticae
Type:
Expression profiling by array
Platform:
GPL16890
11 Samples
Download data: TXT
Series
Accession:
GSE80337
ID:
200080337
9.

Whole genome tomato transcriptional response to tomato-adapted and non-adapted Tetranychus urticae herbivory

(Submitter supplied) Generalist arthropod herbivores rapidly adapt to a broad range of host plants. However, the extent of transcriptional reprogramming in the herbivore and its hosts associated with adaptation remains poorly understood. Using the spider mite Tetranychus urticae and tomato as models with available genomic resources, we investigated the reciprocal genome-wide transcriptional changes in both spider mite and tomato as a consequence of mite’s adaptation to tomato We used microarray to assess global gene expression in Solanum lycopersicum cv. more...
Organism:
Solanum lycopersicum
Type:
Expression profiling by array
Platform:
GPL16358
20 Samples
Download data: CEL
Series
Accession:
GSE70375
ID:
200070375
10.

Host plant adaptation of a polyphagous herbivore shapes transcriptome of both herbivore and host

(Submitter supplied) In an experimental evolutionary set-up, we transferred a genetically diverse strain of the spider mite Tetranychus urticae from its common host bean to tomato where replicated populations were allowed to adapt. By sampling the transcriptomes of non-adapted and adaptes mites feeding on bean and tomato, we identified gene-expression changes in the spider mite affiliated with tomato adaptation. Transcriptional analysis revealed that both constitutive gene-expression levels as well as the transcriptional plasticity of genes were affected. more...
Organism:
Tetranychus urticae
Type:
Expression profiling by array
Platform:
GPL16890
12 Samples
Download data: TXT
Series
Accession:
GSE68708
ID:
200068708
11.

Genome wide gene-expression analysis of facultative reproductive diapause in the two-spotted spider mite Tetranychus urticae

(Submitter supplied) Investigating essential physiological processes in diapausing mites by analyzing genome wide gene expression changes using custom-built microarray. We investigated the molecular biology of facultative reproductive diapause in the chelicerate Tetranychus urticae (Acari: Tetranychidae) by analyzing genome-wide gene expression differences in diapausing and non diapausing T. urticae, using an Agilent custom-built two color gene expression microarray. more...
Organism:
Tetranychus urticae
Type:
Expression profiling by array
Platform:
GPL16890
4 Samples
Download data: TXT
Series
Accession:
GSE48858
ID:
200048858
12.

Reciprocal transcriptional responses in the interaction between Arabidopsis thaliana and Tetranychus urticae.

(Submitter supplied) While pathogen-induced immunity is comparatively well characterized, far less is known about plant defense responses to arthropod herbivores. To date, most molecular-genetic studies of plant-arthropod interactions have focused on insects. However, plant-feeding (phytophagous) mites are also pests of diverse plants, and mites induce different patterns of damage to plant tissues than do well-studied insects (e.g., Lepidopteran larvae or aphids). more...
Organism:
Arabidopsis thaliana
Type:
Expression profiling by array
Platform:
GPL16112
28 Samples
Download data: CEL
Series
Accession:
GSE49981
ID:
200049981
13.

Spider mite responses to feeding on Col-0, myb28 myb29 cyp79b2 cyp79b3, and atr1D Arabidopsis plants

(Submitter supplied) Our goal was to investigate the transcriptional response of T. urticae to different Arabidopsis plants with varying levels of indole glucosinolates.
Organism:
Tetranychus urticae
Type:
Expression profiling by array
Platform:
GPL16890
12 Samples
Download data: TXT
Series
Accession:
GSE48771
ID:
200048771
14.

Genome wide gene-expression analysis of the spider mite Tetranychus urticae after long term host transfer from acyanogenic Phaseolus vulgaris cv. 'Prelude' bean plants to cyanogenic Phaseolus lunatus cv. '8078' bean plants

(Submitter supplied) 250 adult T. urticae females from the London strain (grown on acyanogenic P. vulgaris cv. Prelude bean plants) were transferred to cyanogenic P. lunatus cv. 8078 bean plants. Thirty-five generations after the host transfer, total RNA was extracted from mites growing on both bean species (London and London-CYANO strain) and used in in a genome-wide gene expression microarray (Sureprint G3 microarray, Agilent) experiment to assess significantly differentially expressed genes (FC ≥ 2 and FDR-corrected p-value < 0.05) between mites grown on P. more...
Organism:
Tetranychus urticae
Type:
Expression profiling by array
Platform:
GPL16890
4 Samples
Download data: TXT
Series
Accession:
GSE50162
ID:
200050162
15.

Identification of differentially expressed genes between adult males and adult females of the two-spotted spider mite (Tetranychus urticae)

(Submitter supplied) Sex-specific differences in gene expression underlie differences in morphology, behavior, and reproduction. To date, little is known about sex-specific differences in gene expression in spider mites, even though males and females differ markedly in morphology and behavior. In this study, we describe the complement of sex-specific gene expression differences between males and females of the two-spotted spider mite (Tetranychus urticae), an important generalist herbivore that is a significant crop pest. more...
Organism:
Tetranychus urticae
Type:
Expression profiling by high throughput sequencing
Platform:
GPL21813
8 Samples
Download data: TXT
Series
Accession:
GSE85813
ID:
200085813
16.

Transcriptomic responses in the spider mite Tetranychus urticae to bacterial infection

(Submitter supplied) Genome annotation of the chelicerate Tetranychus urticae revealed the absence of many canonical immunity genes. T. urticae either does not mount an immune response or it induces uncharacterized immune pathways. To disentangle these two hypotheses, we performed transcriptomic analysis of mites injected with bacteria vs mites injected with LB-buffer. Two types of bacteria were injected: E. coli and B. more...
Organism:
Tetranychus urticae
Type:
Expression profiling by array
Platform:
GPL16890
26 Samples
Download data: TXT
Series
Accession:
GSE64199
ID:
200064199
17.

Geographical distribution and molecular insights into abamectin and milbemectin cross-resistance in European field populations of Tetranychus urticae

(Submitter supplied) Macrocylic lactones, milbemectin and abamectin, are compounds that are frequently used for the control of the two-spotted spider mite Tetranychus urticae. The development of abamectin resistance in this major pest has become an increasing problem worldwide, potentially compromising the use of milbemectin. In this study, gene expression profile differences between abamectin/milbemectin resistant T. urticae populations (ES1, IT2 and UK6) and a susceptible T. more...
Organism:
Tetranychus urticae
Type:
Expression profiling by high throughput sequencing
Platforms:
GPL28237 GPL23384
20 Samples
Download data: FASTA, TXT
Series
Accession:
GSE146593
ID:
200146593
18.

Genome sequence of the ectophytic fungus Ramichloridium luteum reveals unique evolutionary adaptations to plant surface niche

(Submitter supplied) We sequenced the complete genome of an ectophytic fungus, Ramichloridium luteum, which colonizes the surface of apple fruit, and carried out comparative genomic and transcriptome analysis
Organism:
Ramichloridium luteum
Type:
Expression profiling by high throughput sequencing
Platform:
GPL23608
6 Samples
Download data: TXT
Series
Accession:
GSE100245
ID:
200100245
19.

Spider mite egg extract modifies Arabidopsis response to future infestations

(Submitter supplied) Transcriptional plant responses are specially under the scope on herbivore oviposition studies. However, most of the information comes from Lepidopteran models, where egg laying and feeding are separate events, and little is known regarding the plant reaction to those pests where females feed and oviposit simultaneously. The present study unveiled the oviposition-induced transcriptomic response of plants to Tetranychus urticae eggs. more...
Organism:
Arabidopsis thaliana
Type:
Expression profiling by high throughput sequencing
Platform:
GPL17639
18 Samples
Download data: TAR
Series
Accession:
GSE168993
ID:
200168993
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