Similar studies for GEO DataSets (Select 200002251) - GEO DataSets

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Items: 20

Select item 2000022511.

Anti-prolif. effect of E2 in breast cancer cells that re-exp. ERalpha is mediated by aberr. regulat. of cell cycle genes

(Submitter supplied) Gene expression changes caused by estrogen treatment of breast cancer cells that re-express ERalpha was investigated by infecting ER-negative MDA-MB-231 breast cancer cells for 24 h with recombinant adenovirus encoding full-length human ERalpha (Ad-ERalpha) or control vector (Ad-LacZ), and treating them with 0·01% ethanol (vehicle control) or 10-8 M 17beta-estradiol (E2). After 48 h of treatment, total RNA was isolated and used for transcript profiling on Affymetrix GeneChips. more...

Organism:: Homo sapiens

Type:: Expression profiling by array

Dataset:: GDS1326
Platform:: GPL96
12 Samples

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Series

Accession:: GSE2251

ID:: 200002251

Select item 13262.

Breast cancer cells reexpressing estrogen receptor alpha response to 17beta-estradiol

Analysis of the response of estrogen receptor (ER) negative MDA-MB-231 breast cancer cells infected with full-length ER alpha adenoviral constructs to treatment with 17beta-estradiol (E2). Results provide insight into the anti-proliferative effect of E2 on breast cancer cells reexpressing ER.

Organism:: Homo sapiens

Type:: Expression profiling by array, count, 2 agent, 2 protocol sets

Platform:: GPL96
Series:: GSE2251
12 Samples

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DataSet

Accession:: GDS1326

ID:: 1326

Select item 2000035293.

Profiling of three different ER+ breast cancer cell lines grown in the presence and absence of estrogen.

(Submitter supplied) Three human ER+ breast cancer cell lines--MCF-7, T47-D, BT-474--grown with or without estradiol (E2). Keywords: Cell Line Comparison

Organism:: Homo sapiens

Type:: Expression profiling by array

Dataset:: GDS1549
Platform:: GPL96
12 Samples

Download data: CEL

Series

Accession:: GSE3529

ID:: 200003529

Select item 15494.

Estrogen effect on estrogen receptor alpha positive breast cancer cell lines

Expression profiling of estrogen receptor positive breast cancer cell lines treated with estradiol for 24 hours. MCF-7, T47-D, and BT-474 breast cancer cell lines examined. Results identify candidate genes involved in estrogen stimulated breast cancer growth.

Organism:: Homo sapiens

Type:: Expression profiling by array, transformed count, 2 agent, 3 cell line sets

Platform:: GPL96
Series:: GSE3529
12 Samples

Download data: CEL

DataSet

Accession:: GDS1549

ID:: 1549

Select item 2000203425.

Estrogen regulation and physiopathologic significance of alternative promoters in breast cancer

(Submitter supplied) Alternative promoters (APs) occur in >30% protein-coding genes and contribute to proteome diversity. However, large-scale analyses of AP regulation are lacking, and little is known about their potential physiopathologic significance. To better understand the transcriptomic impact of estrogens, which play a major role in breast cancer, we analyzed gene and AP regulation by estradiol in MCF7 cells using pan-genomic exon arrays. more...

Organism:: Homo sapiens

Type:: Expression profiling by array

Platforms:: GPL5188 GPL5175
32 Samples

Download data: CEL

Series

Accession:: GSE20342

ID:: 200020342

Select item 2000000416.

Effects of estrogen

(Submitter supplied) Effects of estrogen on global gene expression: identification of novel targets of estrogen action. Keywords: other

Organism:: Homo sapiens

Type:: Expression profiling by SAGE

Platform:: GPL4
4 Samples

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Series

Accession:: GSE41

ID:: 200000041

PubMed Similar studies

Select item 2000115067.

Novel Estrogen Receptor-{alpha} Binding Sites and Estradiol Target Genes Identified by ChIP Cloning in Breast Cancer.

(Submitter supplied) Estrogen receptor-{alpha} (ER{alpha}) and its ligand estradiol play critical roles in breast cancer growth and are important therapeutic targets for this disease. Using chromatin immunoprecipitation (ChIP)-on-chip, ligand-bound ER{alpha} was recently found to function as a master transcriptional regulator via binding to many cis-acting sites genome-wide. Here, we used an alternative technology (ChIP cloning) and identified 94 ER{alpha} target loci in breast cancer cells. more...

Organism:: Homo sapiens

Type:: Expression profiling by array

Dataset:: GDS3283
Platform:: GPL570
9 Samples

Download data: CEL

Series

Accession:: GSE11506

ID:: 200011506

Select item 32838.

Estradiol effect on breast cancer cell line: time course

Analysis of MCF-7 breast cancer cells treated with estradiol for 3 or 6 hours. Results combined with ChIP experiments to identify estrogen receptor alpha binding sites and estradiol target genes in breast cancer cells.

Organism:: Homo sapiens

Type:: Expression profiling by array, transformed count, 2 agent, 2 time sets

Platform:: GPL570
Series:: GSE11506
9 Samples

Download data: CEL

DataSet

Accession:: GDS3283

ID:: 3283

Select item 2000035429.

Profiling of MCF-7 cell lines stably overexpressing (ca)Raf-1, (ca)MEK, (ca)erbB-2, or ligand-activatable EGFR.

(Submitter supplied) Profiling of MCF-7 cell lines stably overexpressing constitutively active Raf-1, constitutively active MEK, constitutively active c-erbB-2, or ligand-activatable EGFR as models of overexpressed growth factor signaling, as well as control vector transfected cells (coMCF-7) and control vector transfected cells long-term adapted for estrogen-independent growth (coMCF-7/lt-E2). Keywords: Cell Line Comparison

Organism:: Homo sapiens

Type:: Expression profiling by array

Dataset:: GDS1925
Platform:: GPL96
18 Samples

Download data: CEL

Series

Accession:: GSE3542

ID:: 200003542

Select item 192510.

Estrogen receptor alpha positive breast cancer cells response to hyperactivation of MAPK pathway

Analysis of estrogen receptor (ER) alpha positive MCF-7 breast cancer cells overexpressing constitutively active Raf-1, constitutively active MEK, constitutively active c-erbB-2, or ligand-activatable EGFR. Results indicate that increased MAPK activation results in loss of ER-alpha expression.

Organism:: Homo sapiens

Type:: Expression profiling by array, count, 6 cell line sets

Platform:: GPL96
Series:: GSE3542
18 Samples

Download data: CEL

DataSet

Accession:: GDS1925

ID:: 1925

Select item 20000400611.

Estrogen effects on MCF-7 breast cancer cells co-expressing ERa and ERb

(Submitter supplied) Two subtypes of the estrogen receptor, ERalpha and ERbeta, mediate the actions of estrogens, and the majority of human breast tumors contain both ERalpha and ERbeta. To examine the possible interactions and modulatory effects of ERbeta on ERalpha activity, we have used adenoviral gene delivery to produce human breast cancer (MCF-7) cells expressing ERbeta, along with their endogenous ERalpha. We have examined the effects of ERβ expression on genome-wide gene expression by Affymetrix GeneChip microarrays. more...

Organism:: Homo sapiens

Type:: Expression profiling by array

Dataset:: GDS2770
Platform:: GPL96
12 Samples

Download data

Series

Accession:: GSE4006

ID:: 200004006

Select item 277012.

Estrogen effect on breast cancer cell line coexpressing estrogen receptors alpha and beta

Analysis of estrogen receptor (ER) alpha positive MCF-7 breast cancer cells following introduction of ERbeta and treatment with estrogen. The majority of breast cancers express both ERalpha and ERbeta. Results provide insight into the comodulatory effects of these two ERs.

Organism:: Homo sapiens

Type:: Expression profiling by array, count, 2 agent, 3 infection sets

Platform:: GPL96
Series:: GSE4006
12 Samples

Download data

DataSet

Accession:: GDS2770

ID:: 2770

Select item 20003668313.

Gene Regulation by Estrogen Signaling and DNA Methylation in MCF7 Breast Cancer Cells

(Submitter supplied) Estrogen signaling and epigenetic modifications, in particular DNA methylation, are involved in regulation of gene expression in breast cancers. Here we investigated a potential regulatory cross-talk between these two pathways by identifying their common target genes and exploring potential underlying molecular mechanisms in human MCF7 breast cancer cells. Principal Findings: Gene expression profiling revealed that the expression of approximately 150 genes was influenced by both 17β-estradiol (E2) and a hypomethylating agent 5-aza-2’-deoxycytidine (DAC). more...

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL13158
12 Samples

Download data: CEL, XLS

Series

Accession:: GSE36683

ID:: 200036683

Select item 20000654814.

An estrogen-dependent model of breast cancer created by transformation of normal human mammary epithelial cells

(Submitter supplied) This study was performed to check that ESR1 and BMI1 are biologically active after lentiviral transduction of primary human mammary epithelial cells (HMECs) with lentiviral vectors expressing ESR1 and BMI1 from the human PGK promoter. ESR1 targets like PGR, PRLR and GREB1, but not TFF1 and XBP1, were induced by estradiol in the ESR1-expressing cells. BMI1 targets like BMI1, NEFL and CCND2 were repressed in the BMI1-expressing cells. more...

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL570
8 Samples

Download data: CEL

Series

Accession:: GSE6548

ID:: 200006548

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20018243115.

Overlapping and distinct functions between ERα and ERβ homodimers and corresponding transcriptomes in the same cellular context

(Submitter supplied) The two estrogen receptors, ERα and ERβ function as ligand-inducible transcription factors. Most in vitro studies have reported that ERα drives breast cancer growth whereas ERβ, if expressed, suppresses growth. To dissect function and gene expression profile regulated by ERα or ERβ, respectively, we generated a novel cell model expressing only ERβ, by applying CRISPR-cas9 to delete ERα in MCF7 cells with stable Tet-Off-inducible ERβ expression. more...

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL21697
12 Samples

Download data: XLSX

Series

Accession:: GSE182431

ID:: 200182431

PubMed Full text in PMC Similar studies Analyze with GEO2R SRA Run Selector

Select item 20007734516.

Genomic analyses of breast cancer cells: 17β-hydroxysteroid dehydrogenase type 1 induces transcriptional changes in estradiol-dependent and independent manners

(Submitter supplied) 17β-HSD1 expression modulates T47D transcript profile in in steroid-deprived medium. The enzyme specifically regulates apoptosis and cancer-related genes in T47D cells cultured in steroid-deprived medium. Genes that primarily involved in Cell cycle progression, such as the Cyclin A2 gene, CCNA2, are generally down-regulated whereas most genes involved in apoptosis and cell death, such as the proapoptotic gene XAF1 and FGF12, are on the contrary up-regulated by 17β-HSD1 knockdown, and 21% of the modulated genes belong to this latter functional category. more...

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL6244
8 Samples

Download data: CEL

Series

Accession:: GSE77345

ID:: 200077345

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20003267017.

Time-course effect of estradiol and estradiol-BSA on early gene expression

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL570
38 Samples

Download data: CEL

Series

Accession:: GSE32670

ID:: 200032670

Select item 20003266918.

Time-course effect of estradiol and estradiol-BSA on early gene expression in SKBR3 cells

(Submitter supplied) Estrogens have been reported to activate several processes via membrane binding to either classic estrogen receptors (ERs) or GPR30. We have used either estradiol or BSA-conjugated estradiol in order to initiate membrane-initiated actions and ICI 172,780 (ICI) or G15 to explore ER- and GPR30-related transcription. Our results show that the majority of G15-inhibited transcription is depending on ERs, as it is also inhibited by ICI. more...