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| Status |
Public on Jan 01, 2012 |
| Title |
Time-course effect of estradiol and estradiol-BSA on early gene expression in MCF-7 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Estrogens have been reported to activate several processes via membrane binding to either classic estrogen receptors (ERs) or GPR30. We have used either estradiol or BSA-conjugated estradiol in order to initiate membrane-initiated actions and ICI 172,780 (ICI) or G15 to explore ER- and GPR30-related transcription. Our results show that the majority of G15-inhibited transcription is depending on ERs, as it is also inhibited by ICI. However, a small number of transcripts, related to specific actions/pathways is either exclusively inhibited by G15, providing evidence about a specific GPR30 signature, or not inhibited by ICI or G15 suggesting the existence of another, yet unidentified estrogen receptor.
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| Overall design |
Cells after a 4h incubation with medium containing 10% charcoal stripped FBS were incubated with or without E2-BSA (10-6M) in the presence or absence of specific antagonists, in DMEM/F12 (1:1) supplemented with 10% charcoal stripped FBS, for 3 hours. Total RNA was isolated using Nucleospin II columns (Macheray-Nagel, Dttren, Germany), according to the manufacturer’s instructions. RNA was labeled and hybridized according to the Affymetrix protocol (Affymetrix Gene-Chip Expression Analysis Technical Manual), using the HGU133A plus 2 chip, analyzing a total of 54675 transcripts. Signals were detected by an Affymetrix microarray chip reader.
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| Contributor(s) |
Notas G, Kampa M, Castanas E |
| Citation(s) |
22406407, 22138208, 22198466 |
| |
| Submission date |
Oct 06, 2011 |
| Last update date |
Mar 25, 2019 |
| Contact name |
George Notas |
| E-mail(s) |
gnotas@uoc.gr
|
| Organization name |
University of Crete
|
| Department |
Medicine
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| Lab |
Experimental Endocrinology
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| Street address |
PO BOX 2208
|
| City |
Heraklion |
| ZIP/Postal code |
71003 |
| Country |
Greece |
| |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE32670 |
Time-course effect of estradiol and estradiol-BSA on early gene expression |
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| Relations |
| BioProject |
PRJNA154469 |
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