NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE6548 Query DataSets for GSE6548
Status Public on Jun 22, 2007
Title An estrogen-dependent model of breast cancer created by transformation of normal human mammary epithelial cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary This study was performed to check that ESR1 and BMI1 are biologically active after lentiviral transduction of primary human mammary epithelial cells (HMECs) with lentiviral vectors expressing ESR1 and BMI1 from the human PGK promoter. ESR1 targets like PGR, PRLR and GREB1, but not TFF1 and XBP1, were induced by estradiol in the ESR1-expressing cells. BMI1 targets like BMI1, NEFL and CCND2 were repressed in the BMI1-expressing cells. BMI1 suppressed genes associated with squamous and neural differentiation in the ESR1 plus BMI1-expressing cells.
Keywords: Drug response
 
Overall design Infect with lentiviruses 24 hours after reduction mammmoplasty. Grow cells in human mammosphere medium: Hepes-buffered DMEM/F12 supplemented with 20 ng/ml EGF, 1x B-27 (GIBCO) plus 1 nM 17-β-estradiol or 1 uM fulvestrant (ICI 182,780).
Lentiviral vectors: pSD-82, ESR1; pSD-84, BMI1; pSD-86, gusA.
 
Contributor(s) Duss S, André S, Nicoulaz A, Fiche M, Bonnefoi H, Brisken C, Iggo R
Citation(s) 17573968
Submission date Dec 16, 2006
Last update date Mar 25, 2019
Contact name Richard Iggo
E-mail(s) r.iggo@bordeaux.unicancer.fr
Phone +33 5 56 33 04 23
Fax +33 5 56 33 32 06
Organization name University of Bordeaux
Department Bergonie Cancer Institute
Lab INSERM U916
Street address 229 cours de l'Argonne
City Bordeaux
ZIP/Postal code 33076
Country France
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (8)
GSM151612 pSD-82 + E2
GSM151623 pSD-82 + F
GSM151624 pSD-84 + E2
Relations
BioProject PRJNA98591

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE6548_RAW.tar 71.3 Mb (http)(custom) TAR (of CEL)

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap