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Series GSE51629 Query DataSets for GSE51629
Status Public on Oct 24, 2013
Title Treatment of prostate cancer cell lines DU-145 and LNCaP with zebularine
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Cultures of DU-145 cells and of LNCaP cells were treated for 216 hours with 100µM zebularine (SIGMA) in three independent biological experiments. Zebularine acts as a DNA methyltransferase (DNMT) inhibitor thereby upregulating genes that are inactivated by e.g. promotor hypermethylation. The experiment aimed to search for upregulated transcripts to provide new targets for biomarker development and therapeutic use.
Overall design Total RNA of untreated and treated (100µM zebularine) DU-145 cells (experiment HK_21_DU_145-HK_26_DU_145), and of untreated and treated (100µM zebularine) LNCaP cells (experiment HK_27_LNCaP-HK_32_LNCaP), were subjected to Affymetrix array analysis to detail the overall expression changes after treatment with a DNMT inhibitor. Treated cells showed no obvious signs of zebularine-induced cytotoxicity as revealed by XTT assays.
Contributor(s) Krause HG, Ikromov O
Citation Odiljon Ikromov, Imad Alkamal, AhmedMagheli, Nadine Ratert, Mauricio Sendeski, Kurt Miller, Hans Krause, and Carsten Kempkensteffen. Functional Epigenetic Analysis of Prostate Carcinoma: A Role for Seryl-tRNA Synthetase? Hindawi Publishing Corporation, Journal of Biomarkers Volume 2014, Article ID 362164, 10 pages.
Submission date Oct 23, 2013
Last update date Jan 25, 2019
Contact name Hans G Krause
Organization name Charité - Universitätsmedizin Berlin
Department Klinik für Urologie
Street address Charitéplatz 1
City Berlin
ZIP/Postal code 10117
Country Germany
Platforms (1)
GPL10739 [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [probe set (exon) version]
Samples (12)
GSM1249704 Untreated control, DU-145, biological rep 1
GSM1249705 100µM zebularine, DU-145, biological rep 1
GSM1249706 Untreated control, DU-145, biological rep 2
BioProject PRJNA224117

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE51629_RAW.tar 49.0 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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