GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM1249707 Query DataSets for GSM1249707
Status Public on Oct 24, 2013
Title 100µM zebularine, DU-145, biological rep 2
Sample type RNA
Source name DU-145 cells, 100µM zebularine, 216hours
Organism Homo sapiens
Characteristics cell line: DU-145
cell type: prostate cancer cell line
treatment: 100µM zebularine
time: 216 hrs
Treatment protocol Cell seeding (day0), treatment start (24 hours later, day1), medium change and splitting of cells on day3 and day6.
Growth protocol Monolayer cell culture (RPMI-1640 + 10%FCS) in the presence (100µM) or absence of Zebularine (SIGMA, cat. no. Z4775).
Extracted molecule total RNA
Extraction protocol miRNeasy Mini Kit Qiagen (Cat. No.217004) total RNA extraction was done according to manufacturer's instructions, QC by AGILENT Bioanalyzer (only RIN >8.0 used).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 300 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, cRNAs were hybridized for 16 hr at 45C on GeneChip Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned on the GeneChip Scanner 3000 G7.
Description HK_24_DU_145_treat_2
ATCC No.: HTB-81
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Submission date Oct 23, 2013
Last update date Jan 24, 2014
Contact name Hans G Krause
Organization name Charité - Universitätsmedizin Berlin
Department Klinik für Urologie
Street address Charitéplatz 1
City Berlin
ZIP/Postal code 10117
Country Germany
Platform ID GPL10739
Series (1)
GSE51629 Treatment of prostate cancer cell lines DU-145 and LNCaP with zebularine

Data table header descriptions
VALUE MAS5.0 signal intensity (log2 transformed)

Data table
7892501 7.92227
7892502 4.98402
7892503 4.58448
7892504 9.99303
7892505 4.86311
7892506 4.52222
7892507 5.55507
7892508 7.08589
7892509 11.9766
7892510 3.0876
7892511 4.09823
7892512 7.77206
7892513 3.38208
7892514 11.143
7892515 9.71618
7892516 6.84771
7892517 5.85891
7892518 3.96724
7892519 6.07196
7892520 8.60808

Total number of rows: 257101

Table truncated, full table size 3989 Kbytes.

Supplementary file Size Download File type/resource
GSM1249707_HK_24_DU_145_treat_2.CEL.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap