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Sample GSM1249706 Query DataSets for GSM1249706
Status Public on Oct 24, 2013
Title Untreated control, DU-145, biological rep 2
Sample type RNA
 
Source name DU-145 cells, 0µM zebularine, 216hours
Organism Homo sapiens
Characteristics cell line: DU-145
cell type: prostate cancer cell line
treatment: none
time: 216 hrs
Treatment protocol Cell seeding (day0), treatment start (24 hours later, day1), medium change and splitting of cells on day3 and day6.
Growth protocol Monolayer cell culture (RPMI-1640 + 10%FCS) in the presence (100µM) or absence of Zebularine (SIGMA, cat. no. Z4775).
Extracted molecule total RNA
Extraction protocol miRNeasy Mini Kit Qiagen (Cat. No.217004) total RNA extraction was done according to manufacturer's instructions, QC by AGILENT Bioanalyzer (only RIN >8.0 used).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 300 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, cRNAs were hybridized for 16 hr at 45C on GeneChip Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned on the GeneChip Scanner 3000 G7.
Description HK_23_DU_145_mock_2
ATCC No.: HTB-81
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Oct 23, 2013
Last update date Jan 24, 2014
Contact name Hans G Krause
E-mail(s) hans.krause@charite.de
Organization name Charité - Universitätsmedizin Berlin
Department Klinik für Urologie
Street address Charitéplatz 1
City Berlin
ZIP/Postal code 10117
Country Germany
 
Platform ID GPL10739
Series (1)
GSE51629 Treatment of prostate cancer cell lines DU-145 and LNCaP with zebularine

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity (log2 transformed)

Data table
ID_REF VALUE
7892501 4.24214
7892502 4.86652
7892503 3.63335
7892504 9.53691
7892505 4.33154
7892506 5.04059
7892507 5.05424
7892508 5.99214
7892509 11.7105
7892510 4.39072
7892511 2.86305
7892512 7.59907
7892513 3.59809
7892514 10.9835
7892515 9.51723
7892516 5.42662
7892517 6.09419
7892518 3.57729
7892519 6.0358
7892520 9.20158

Total number of rows: 257101

Table truncated, full table size 3991 Kbytes.




Supplementary file Size Download File type/resource
GSM1249706_HK_23_DU_145_mock_2.CEL.gz 4.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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