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Sample GSM1249711 Query DataSets for GSM1249711
Status Public on Oct 24, 2013
Title 100µM zebularine, LNCaP, biological rep 1
Sample type RNA
 
Source name LNCaP cells, 100µM zebularine, 216hours
Organism Homo sapiens
Characteristics cell line: LNCaP
cell type: prostate cancer cell line
treatment: 100µM zebularine
time: 216 hrs
Treatment protocol Cell seeding (day0), treatment start (24 hours later, day1), medium change and splitting of cells on day3 and day6.
Growth protocol Monolayer cell culture (RPMI-1640 + 10%FCS) in the presence (100µM) or absence of Zebularine (SIGMA, cat. no. Z4775).
Extracted molecule total RNA
Extraction protocol miRNeasy Mini Kit Qiagen (Cat. No.217004) total RNA extraction was done according to manufacturer's instructions, QC by AGILENT Bioanalyzer (only RIN >8.0 used).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 300 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, cRNAs were hybridized for 16 hr at 45C on GeneChip Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned on the GeneChip Scanner 3000 G7.
Description HK_28_LNCaP_treat_1
ATCC No.: HTB-1740
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Oct 23, 2013
Last update date Jan 24, 2014
Contact name Hans G Krause
E-mail(s) hans.krause@charite.de
Organization name Charité - Universitätsmedizin Berlin
Department Klinik für Urologie
Street address Charitéplatz 1
City Berlin
ZIP/Postal code 10117
Country Germany
 
Platform ID GPL10739
Series (1)
GSE51629 Treatment of prostate cancer cell lines DU-145 and LNCaP with zebularine

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity (log2 transformed)

Data table
ID_REF VALUE
7892501 7.12577
7892502 5.05173
7892503 3.28421
7892504 9.84922
7892505 4.30107
7892506 5.46714
7892507 5.65626
7892508 7.20772
7892509 11.4422
7892510 3.92922
7892511 3.32536
7892512 7.79328
7892513 4.0663
7892514 11.4695
7892515 10.0014
7892516 6.27434
7892517 6.90279
7892518 3.32305
7892519 6.26398
7892520 9.30146

Total number of rows: 257101

Table truncated, full table size 3991 Kbytes.




Supplementary file Size Download File type/resource
GSM1249711_HK_28_LNCaP_treat_1.CEL.gz 4.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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