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Sample GSM1249710 Query DataSets for GSM1249710
Status Public on Oct 24, 2013
Title Untreated control, LNCaP, biological rep 1
Sample type RNA
 
Source name LNCaP cells, 0µM zebularine, 216hours
Organism Homo sapiens
Characteristics cell line: LNCaP
cell type: prostate cancer cell line
treatment: none
time: 216 hrs
Treatment protocol Cell seeding (day0), treatment start (24 hours later, day1), medium change and splitting of cells on day3 and day6.
Growth protocol Monolayer cell culture (RPMI-1640 + 10%FCS) in the presence (100µM) or absence of Zebularine (SIGMA, cat. no. Z4775).
Extracted molecule total RNA
Extraction protocol miRNeasy Mini Kit Qiagen (Cat. No.217004) total RNA extraction was done according to manufacturer's instructions, QC by AGILENT Bioanalyzer (only RIN >8.0 used).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 300 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, cRNAs were hybridized for 16 hr at 45C on GeneChip Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned on the GeneChip Scanner 3000 G7.
Description HK_27_LNCaP_mock_1
ATCC No.: HTB-1740
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Oct 23, 2013
Last update date Jan 24, 2014
Contact name Hans G Krause
E-mail(s) hans.krause@charite.de
Organization name Charité - Universitätsmedizin Berlin
Department Klinik für Urologie
Street address Charitéplatz 1
City Berlin
ZIP/Postal code 10117
Country Germany
 
Platform ID GPL10739
Series (1)
GSE51629 Treatment of prostate cancer cell lines DU-145 and LNCaP with zebularine

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity (log2 transformed)

Data table
ID_REF VALUE
7892501 7.69293
7892502 5.92294
7892503 4.62549
7892504 9.42613
7892505 4.79268
7892506 5.34386
7892507 5.72463
7892508 7.62078
7892509 11.6523
7892510 4.00103
7892511 4.36594
7892512 7.57172
7892513 3.93155
7892514 11.1772
7892515 9.71215
7892516 6.35399
7892517 7.01132
7892518 4.30134
7892519 5.84629
7892520 9.14103

Total number of rows: 257101

Table truncated, full table size 3989 Kbytes.




Supplementary file Size Download File type/resource
GSM1249710_HK_27_LNCaP_mock_1.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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