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Sample GSM1249708 Query DataSets for GSM1249708
Status Public on Oct 24, 2013
Title Untreated control, DU-145, biological rep 3
Sample type RNA
 
Source name DU-145 cells, 0µM zebularine, 216hours
Organism Homo sapiens
Characteristics cell line: DU-145
cell type: prostate cancer cell line
treatment: none
time: 216 hrs
Treatment protocol Cell seeding (day0), treatment start (24 hours later, day1), medium change and splitting of cells on day3 and day6.
Growth protocol Monolayer cell culture (RPMI-1640 + 10%FCS) in the presence (100µM) or absence of Zebularine (SIGMA, cat. no. Z4775).
Extracted molecule total RNA
Extraction protocol miRNeasy Mini Kit Qiagen (Cat. No.217004) total RNA extraction was done according to manufacturer's instructions, QC by AGILENT Bioanalyzer (only RIN >8.0 used).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 300 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, cRNAs were hybridized for 16 hr at 45C on GeneChip Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned on the GeneChip Scanner 3000 G7.
Description HK_25_DU_145_mock_3
ATCC No.: HTB-81
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Oct 23, 2013
Last update date Jan 24, 2014
Contact name Hans G Krause
E-mail(s) hans.krause@charite.de
Organization name Charité - Universitätsmedizin Berlin
Department Klinik für Urologie
Street address Charitéplatz 1
City Berlin
ZIP/Postal code 10117
Country Germany
 
Platform ID GPL10739
Series (1)
GSE51629 Treatment of prostate cancer cell lines DU-145 and LNCaP with zebularine

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity (log2 transformed)

Data table
ID_REF VALUE
7892501 7.09941
7892502 5.35545
7892503 4.26058
7892504 9.57006
7892505 4.29238
7892506 5.48549
7892507 5.79752
7892508 6.59725
7892509 12.0579
7892510 4.53773
7892511 3.86458
7892512 7.31011
7892513 3.38788
7892514 11.3508
7892515 9.70007
7892516 5.38898
7892517 6.65414
7892518 4.95058
7892519 5.37391
7892520 9.03758

Total number of rows: 257101

Table truncated, full table size 3990 Kbytes.




Supplementary file Size Download File type/resource
GSM1249708_HK_25_DU_145_mock_3.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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