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Series GSE45384 Query DataSets for GSE45384
Status Public on Dec 31, 2013
Title Effects of Th1- and Th17-derived factors on microglia.
Organism Mus musculus
Experiment type Expression profiling by array
Summary There is evidence that microglia interact with infiltrating Th1 and Th17 cells and this interaction results in mutual activation. However, the potential of a distinct cytokine milieu generated by these effector T cell subsets to activate microglia is poorly understood. In this study, we tested the ability of factors secreted by Th1 and Th17 cells to induce microglial activation. Interestingly, we found that only Th1-associated factors had the potential to activate microglia while the Th17-associated factors as well as direct contact of Th17 cells with microglia only had a minimal effect. Further Th1-derived factors triggered a proinflammatory M1-type gene expression profile in microglia
Overall design Microglia harvested from mixed glial cultures were treated with supernatants from Th1- or Th17 cultures. Microglia cultured in medium was used as controls. At 16h post treatment RNA was isolated from the microglia and probed on AgilentĀ“s murine 4x44k microarrays. RNA isolated from four independent experiments were used for the gene expression profiling.
Microglia, Th1, Th17
Contributor(s) Geffers R, Floess S, Huehn J
Citation(s) 30364000
Submission date Mar 21, 2013
Last update date Oct 29, 2018
Contact name Robert Geffers
Phone +49 531-6181-3058
Organization name HCI - Helmholtz Centre for Infection Research
Department Dep. Molecular Bacteriology
Lab Genome Analytics
Street address Inhoffenstr. 7
City Braunschweig
ZIP/Postal code 38124
Country Germany
Platforms (1)
GPL11202 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version)
Samples (12)
GSM1103441 12-1_IMDM
GSM1103442 12-2_Th1
GSM1103443 12-3_Th17
BioProject PRJNA193605

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE45384_RAW.tar 25.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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