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Sample GSM1103452 Query DataSets for GSM1103452
Status Public on Dec 31, 2013
Title 16-3_Th17
Sample type RNA
Source name Th17 supernatant
Organism Mus musculus
Characteristics treatment: Th17 supernatant
cell type: microglia
Treatment protocol Th17 supernatant
Extracted molecule total RNA
Extraction protocol RNAeasy
Label Cy3
Label protocol 500ng of total RNA were applied for Cy3-labelling reaction using the one color Quick Amp Labeling protocol (Agilent Technologies; Waldbronn, Germany).
Hybridization protocol 1.6 µg labeled cRNA was hybridized to microarrays for 16h at 68°C and scanned using the Agilent DNA Microarray Scanner.
Scan protocol Microarrays were scanned using the Agilent Scanner System. Data extractions were performed using Feature Extraction Software 10.7.1 (Agilent) with default patrameters of the GE1_107_Sep09 extraction protocol
Description 16-3_Th17
Data processing log2 transformed, median normalized to all samples, signal intensity expressed as relative to median signal intensity of all samples
Submission date Mar 21, 2013
Last update date Apr 25, 2018
Contact name Robert Geffers
Phone +49 531-6181-3058
Organization name HCI - Helmholtz Centre for Infection Research
Department Dep. Molecular Bacteriology
Lab Genome Analytics
Street address Inhoffenstr. 7
City Braunschweig
ZIP/Postal code 38124
Country Germany
Platform ID GPL11202
Series (1)
GSE45384 Effects of Th1- and Th17-derived factors on microglia.
Reanalyzed by GSE113579

Data table header descriptions
VALUE median centered relative expression signal

Data table
A_55_P1989846 -0.1861372
A_55_P1991598 -0.07747269
A_55_P2022211 -0.6381564
A_55_P1980764 -0.82760334
A_55_P1964375 -0.002016544
A_51_P128876 -0.533854
A_55_P2121042 0.033629894
A_52_P219230 -0.54259014
A_51_P207591 0.11432171
A_55_P2131920 -0.020477295
A_55_P2404223 -0.01696825
A_55_P2101944 -0.09065008
A_52_P358860 0.19114637
A_51_P119031 -0.036486626
A_51_P309854 0.16967678
A_51_P343900 0.085436344
A_51_P234359 -0.12265158
A_51_P487813 0.18907595
A_52_P613977 -0.15145063
A_55_P1957209 0.17607355

Total number of rows: 39483

Table truncated, full table size 960 Kbytes.

Supplementary file Size Download File type/resource
GSM1103452_Floess1012.txt.gz 2.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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