|
| Status |
Public on Dec 31, 2013 |
| Title |
16-3_Th17 |
| Sample type |
RNA |
| |
|
| Source name |
Th17 supernatant
|
| Organism |
Mus musculus |
| Characteristics |
treatment: Th17 supernatant
cell type: microglia
|
| Treatment protocol |
Th17 supernatant
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNAeasy
|
| Label |
Cy3
|
| Label protocol |
500ng of total RNA were applied for Cy3-labelling reaction using the one color Quick Amp Labeling protocol (Agilent Technologies; Waldbronn, Germany).
|
| |
|
| Hybridization protocol |
1.6 µg labeled cRNA was hybridized to microarrays for 16h at 68°C and scanned using the Agilent DNA Microarray Scanner.
|
| Scan protocol |
Microarrays were scanned using the Agilent Scanner System. Data extractions were performed using Feature Extraction Software 10.7.1 (Agilent) with default patrameters of the GE1_107_Sep09 extraction protocol
|
| Description |
16-3_Th17
|
| Data processing |
log2 transformed, median normalized to all samples, signal intensity expressed as relative to median signal intensity of all samples
|
| |
|
| Submission date |
Mar 21, 2013 |
| Last update date |
Apr 25, 2018 |
| Contact name |
Robert Geffers |
| E-mail(s) |
robert.geffers@helmholtz-hzi.de
|
| Phone |
+49 531-6181-3058
|
| Organization name |
HCI - Helmholtz Centre for Infection Research
|
| Department |
Dep. Molecular Bacteriology
|
| Lab |
Genome Analytics
|
| Street address |
Inhoffenstr. 7
|
| City |
Braunschweig |
| ZIP/Postal code |
38124 |
| Country |
Germany |
| |
|
| Platform ID |
GPL11202 |
| Series (1) |
| GSE45384 |
Effects of Th1- and Th17-derived factors on microglia. |
|
| Relations |
| Reanalyzed by |
GSE113579 |
