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Sample GSM1103445 Query DataSets for GSM1103445
Status Public on Dec 31, 2013
Title 13-2_Th1
Sample type RNA
Source name Th1 supernatant
Organism Mus musculus
Characteristics treatment: Th1 supernatant
cell type: microglia
Treatment protocol Th1 supernatant
Extracted molecule total RNA
Extraction protocol RNAeasy
Label Cy3
Label protocol 500ng of total RNA were applied for Cy3-labelling reaction using the one color Quick Amp Labeling protocol (Agilent Technologies; Waldbronn, Germany).
Hybridization protocol 1.6 µg labeled cRNA was hybridized to microarrays for 16h at 68°C and scanned using the Agilent DNA Microarray Scanner.
Scan protocol Microarrays were scanned using the Agilent Scanner System. Data extractions were performed using Feature Extraction Software 10.7.1 (Agilent) with default patrameters of the GE1_107_Sep09 extraction protocol
Description 13-2_Th1
Data processing log2 transformed, median normalized to all samples, signal intensity expressed as relative to median signal intensity of all samples
Submission date Mar 21, 2013
Last update date Apr 25, 2018
Contact name Robert Geffers
Phone +49 531-6181-3058
Organization name HCI - Helmholtz Centre for Infection Research
Department Dep. Molecular Bacteriology
Lab Genome Analytics
Street address Inhoffenstr. 7
City Braunschweig
ZIP/Postal code 38124
Country Germany
Platform ID GPL11202
Series (1)
GSE45384 Effects of Th1- and Th17-derived factors on microglia.
Reanalyzed by GSE113579

Data table header descriptions
VALUE median centered relative expression signal

Data table
A_55_P1989846 0.52347374
A_55_P1991598 -0.3977604
A_55_P2022211 0.10911131
A_55_P1980764 0.32975817
A_55_P1964375 -0.1171813
A_51_P128876 2.5261302
A_55_P2121042 -0.2732401
A_52_P219230 0.77449226
A_51_P207591 0.3466568
A_55_P2131920 -0.31827497
A_55_P2404223 -0.047314167
A_55_P2101944 -0.53913164
A_52_P358860 -0.41248655
A_51_P119031 -0.46315002
A_51_P309854 -0.0581007
A_51_P343900 0.059445858
A_51_P234359 1.1839352
A_51_P487813 -0.48263502
A_52_P613977 0.42601633
A_55_P1957209 -0.07678795

Total number of rows: 39483

Table truncated, full table size 962 Kbytes.

Supplementary file Size Download File type/resource
GSM1103445_Floess1005.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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