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Sample GSM1103443 Query DataSets for GSM1103443
Status Public on Dec 31, 2013
Title 12-3_Th17
Sample type RNA
 
Source name Th17 supernatant
Organism Mus musculus
Characteristics treatment: Th17 supernatant
cell type: microglia
Treatment protocol Th17 supernatant
Extracted molecule total RNA
Extraction protocol RNAeasy
Label Cy3
Label protocol 500ng of total RNA were applied for Cy3-labelling reaction using the one color Quick Amp Labeling protocol (Agilent Technologies; Waldbronn, Germany).
 
Hybridization protocol 1.6 µg labeled cRNA was hybridized to microarrays for 16h at 68°C and scanned using the Agilent DNA Microarray Scanner.
Scan protocol Microarrays were scanned using the Agilent Scanner System. Data extractions were performed using Feature Extraction Software 10.7.1 (Agilent) with default patrameters of the GE1_107_Sep09 extraction protocol
Description 12-3_Th17
Data processing log2 transformed, median normalized to all samples, signal intensity expressed as relative to median signal intensity of all samples
 
Submission date Mar 21, 2013
Last update date Apr 25, 2018
Contact name Robert Geffers
E-mail(s) robert.geffers@helmholtz-hzi.de
Phone +49 531-6181-3058
Organization name HCI - Helmholtz Centre for Infection Research
Department Dep. Molecular Bacteriology
Lab Genome Analytics
Street address Inhoffenstr. 7
City Braunschweig
ZIP/Postal code 38124
Country Germany
 
Platform ID GPL11202
Series (1)
GSE45384 Effects of Th1- and Th17-derived factors on microglia.
Relations
Reanalyzed by GSE113579

Data table header descriptions
ID_REF
VALUE median centered relative expression signal

Data table
ID_REF VALUE
A_55_P1989846 -0.01749897
A_55_P1991598 -0.2778511
A_55_P2022211 -0.053267956
A_55_P1980764 0.37996626
A_55_P1964375 0.002016544
A_51_P128876 -1.749701
A_55_P2121042 -0.1738081
A_52_P219230 -0.07958603
A_51_P207591 -0.03563881
A_55_P2131920 -0.23679495
A_55_P2404223 0.01696825
A_55_P2101944 0.48685408
A_52_P358860 0.1528039
A_51_P119031 0.121790886
A_51_P309854 -0.16511917
A_51_P343900 0.027367115
A_51_P234359 -0.062167168
A_51_P487813 0.19221735
A_52_P613977 -0.10068941
A_55_P1957209 -0.08756256

Total number of rows: 39483

Table truncated, full table size 969 Kbytes.




Supplementary file Size Download File type/resource
GSM1103443_Floess1003.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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