NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1103442 Query DataSets for GSM1103442
Status Public on Dec 31, 2013
Title 12-2_Th1
Sample type RNA
 
Source name Th1 supernatant
Organism Mus musculus
Characteristics treatment: Th1 supernatant
cell type: microglia
Treatment protocol Th1 supernatant
Extracted molecule total RNA
Extraction protocol RNAeasy
Label Cy3
Label protocol 500ng of total RNA were applied for Cy3-labelling reaction using the one color Quick Amp Labeling protocol (Agilent Technologies; Waldbronn, Germany).
 
Hybridization protocol 1.6 µg labeled cRNA was hybridized to microarrays for 16h at 68°C and scanned using the Agilent DNA Microarray Scanner.
Scan protocol Microarrays were scanned using the Agilent Scanner System. Data extractions were performed using Feature Extraction Software 10.7.1 (Agilent) with default patrameters of the GE1_107_Sep09 extraction protocol
Description 12-2_Th1
Data processing log2 transformed, median normalized to all samples, signal intensity expressed as relative to median signal intensity of all samples
 
Submission date Mar 21, 2013
Last update date Apr 25, 2018
Contact name Robert Geffers
E-mail(s) robert.geffers@helmholtz-hzi.de
Phone +49 531-6181-3058
Organization name HCI - Helmholtz Centre for Infection Research
Department Dep. Molecular Bacteriology
Lab Genome Analytics
Street address Inhoffenstr. 7
City Braunschweig
ZIP/Postal code 38124
Country Germany
 
Platform ID GPL11202
Series (1)
GSE45384 Effects of Th1- and Th17-derived factors on microglia.
Relations
Reanalyzed by GSE113579

Data table header descriptions
ID_REF
VALUE median centered relative expression signal

Data table
ID_REF VALUE
A_55_P1989846 0.40253258
A_55_P1991598 0.748724
A_55_P2022211 0.17549276
A_55_P1980764 0.31281805
A_55_P1964375 -0.021415234
A_51_P128876 2.9626317
A_55_P2121042 -0.033629417
A_52_P219230 0.41903782
A_51_P207591 0.21966028
A_55_P2131920 -0.107978344
A_55_P2404223 0.32709455
A_55_P2101944 -0.7475009
A_52_P358860 -0.38214922
A_51_P119031 -0.53912354
A_51_P309854 -0.13371849
A_51_P343900 0.044287205
A_51_P234359 1.7063389
A_51_P487813 -0.029268742
A_52_P613977 0.051938534
A_55_P1957209 -0.04392624

Total number of rows: 39483

Table truncated, full table size 957 Kbytes.




Supplementary file Size Download File type/resource
GSM1103442_Floess1002.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap