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Sample GSM1103449 Query DataSets for GSM1103449
Status Public on Dec 31, 2013
Title 14-3_Th17
Sample type RNA
Source name Th17 supernatant
Organism Mus musculus
Characteristics treatment: Th17 supernatant
cell type: microglia
Treatment protocol Th17 supernatant
Extracted molecule total RNA
Extraction protocol RNAeasy
Label Cy3
Label protocol 500ng of total RNA were applied for Cy3-labelling reaction using the one color Quick Amp Labeling protocol (Agilent Technologies; Waldbronn, Germany).
Hybridization protocol 1.6 µg labeled cRNA was hybridized to microarrays for 16h at 68°C and scanned using the Agilent DNA Microarray Scanner.
Scan protocol Microarrays were scanned using the Agilent Scanner System. Data extractions were performed using Feature Extraction Software 10.7.1 (Agilent) with default patrameters of the GE1_107_Sep09 extraction protocol
Description 14-3_Th17
Data processing log2 transformed, median normalized to all samples, signal intensity expressed as relative to median signal intensity of all samples
Submission date Mar 21, 2013
Last update date Apr 25, 2018
Contact name Robert Geffers
Phone +49 531-6181-3058
Organization name HCI - Helmholtz Centre for Infection Research
Department Dep. Molecular Bacteriology
Lab Genome Analytics
Street address Inhoffenstr. 7
City Braunschweig
ZIP/Postal code 38124
Country Germany
Platform ID GPL11202
Series (1)
GSE45384 Effects of Th1- and Th17-derived factors on microglia.
Reanalyzed by GSE113579

Data table header descriptions
VALUE median centered relative expression signal

Data table
A_55_P1989846 -0.48233318
A_55_P1991598 1.0085483
A_55_P2022211 -0.015890598
A_55_P1980764 -0.6080885
A_55_P1964375 0.003201008
A_51_P128876 -0.28379107
A_55_P2121042 0.52500725
A_52_P219230 -0.57039165
A_51_P207591 -0.11584091
A_55_P2131920 0.47017527
A_55_P2404223 0.018991947
A_55_P2101944 1.1056991
A_52_P358860 0.3706956
A_51_P119031 0.10270023
A_51_P309854 0.5287242
A_51_P343900 -0.06363726
A_51_P234359 0.42750788
A_51_P487813 0.09750605
A_52_P613977 -0.19696283
A_55_P1957209 0.64639854

Total number of rows: 39483

Table truncated, full table size 956 Kbytes.

Supplementary file Size Download File type/resource
GSM1103449_Floess1009.txt.gz 2.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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