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Series GSE113579 Query DataSets for GSE113579
Status Public on Dec 03, 2018
Title IFN-g producing Th1 cells induce different transcriptional profiles in microglia and astrocytes
Organism Mus musculus
Experiment type Expression profiling by array
Third-party reanalysis
Summary Autoreactive T cells that infiltrate into the central nervous system (CNS) are believed to have a significant role in mediating the pathology of neurodegenerative diseases such as Alzheimer's disease, amyotrophic lateral sclerosis and multiple sclerosis. Their interaction with microglia and astrocytes in the CNS is crucial for the regulation of the neuroinflammatory process. Our previous work demonstrated that effectors secreted by Th1 and Th17 cells have different capacities to influence the phenotype and function of the glial cells. We have shown that Th1 effectors altered the phenotype and function of both microglia and astrocytes whereas Th17 effectors induced direct effects only on astrocytes but not on microglia. Here we investigated if effector molecules associated with IFN-g producing Th1 cells induced different gene expression profiles in microglia and astrocytes. We performed a microarray analysis of RNA isolated from microglia and astrocytes treated with medium and Th1 culture supernatants and compared the gene expression data. By using the criteria of 2-fold change and a false discovery rate of 0.01 (corrected p-value < 0.01), we demonstrated that a total of 2106 and 1594 genes were differentially regulated microglia and astrocytes respectively in response to Th1-derived factors. We observed that Th1 associated effectors induce distinct transcriptional changes in microglia and astrocytes in addition to commonly regulated transcripts. These distinct transcriptional changes regulate distinct physiological functions and this knowledge can help in better understanding of T cell mediated neuropathologies.
Overall design microarray analysis of RNA isolated from astrocytes treated with medium and Th1 culture supernatants

Please note that in order to comparatively analyze this dataset with a previously generated, published microglia dataset [GSE45384], raw data from both studies were reprocessed and normalized together (as detailed in the sample data processing description) and the re-analyzed data is provided as a Series supplementary file:
Contributor(s) Prajeeth CK, Dittrich-Breiholz O, Talbot SR, Huehn J, Stangel M
Citation(s) 30364000
Submission date Apr 24, 2018
Last update date Dec 26, 2018
Contact name Chittappen Kandiyil Prajeeth
Phone 00495115323816
Organization name Hannover Medical School
Department Neurology
Lab Neuroimmunology
Street address Carl-Neuberg Str 1
City Hannover
ZIP/Postal code 30625
Country Germany
Platforms (1)
GPL24917 Agilent-066423 ‘048306On1M’ (Probe Name Version)
Samples (12)
GSM3109262 1-1 Medium [M5313]
GSM3109263 1-2 Th1 [M5314]
GSM3109264 1-3 Th17 [M5315]
Reanalysis of GSM1103441
Reanalysis of GSM1103442
Reanalysis of GSM1103443
Reanalysis of GSM1103444
Reanalysis of GSM1103445
Reanalysis of GSM1103446
Reanalysis of GSM1103447
Reanalysis of GSM1103448
Reanalysis of GSM1103449
Reanalysis of GSM1103450
Reanalysis of GSM1103451
Reanalysis of GSM1103452
BioProject PRJNA453143

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE113579_GSM1103441-GSM1103452_re-analyzed_GSE45384_matrix.txt.gz 762.1 Kb (ftp)(http) TXT
GSE113579_RAW.tar 208.0 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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