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| Status |
Public on Oct 17, 2013 |
| Title |
HIV-1 gp120 impairs B cell proliferation by inducing TGF-β1 production and FcRL4 expression via an α4β7-dependent mechanism |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The anti-HIV humoral immune response following acute infection is delayed and ineffective. HIV envelope protein gp120 binds to and signals through α4β7 on T cells. We show that gp120 also binds and signals through α4β7 on B cells, resulting in an abortive proliferative response. In primary B cells, gp120 signaling through α4β7 resulted in increased expression of TGF-β1 and the B cell inhibitory receptor FcRL4. Co-culture of B cells with HIV-infected autologous CD4+ T cells also resulted in increased B cell FcRL4 expression. These findings indicate that, in addition to inducing chronic immune activation, viral proteins can contribute directly to HIV-associated B cell dysfunction, thus providing a mechanism whereby the virus subverts the early HIV-specific humoral immune response.
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| Overall design |
Forty-two samples in two batches were run with different treatments and different time points.
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| Contributor(s) |
Jelicic K, Nawaz F, Huang DW, Zheng X, Yang J, Lempicki R, Pascuccio M, Van Ryk D, Schwing C, Hiatt J, Wei D, Roby G, Cimbro R, David A, Hwang IY, Kehrl JH, Arthos J, Cicala C, Fauci A |
| Citation(s) |
24162774 |
| Submission date |
Jul 18, 2013 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Richard Lempicki |
| Organization name |
NCI-Frederick
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| Lab |
Laboratory of Immunopathogenesis and Bioinformatics
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| Street address |
1050 Boyles Street / P.O. Box B
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| City |
Frederick |
| State/province |
MD |
| ZIP/Postal code |
21702 |
| Country |
USA |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (42)
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| GSM1192218 |
Bcell_activated-gp120m_30min, AD4 |
| GSM1192219 |
Bcell_activated_3hrs, AD5 |
| GSM1192220 |
Bcell_activated-gp120pp_3hrs, AD6 |
| GSM1192221 |
Bcell_activated-gp120m_3hrs, AD7 |
| GSM1192222 |
Bcell_activated_6hrs, AD8 |
| GSM1192223 |
Bcell_activated-gp120pp_6hrs, AD9 |
| GSM1192224 |
Bcell_activated-gp120m_6hrs, AD10 |
| GSM1192225 |
Bcell_mock_0hrs, AE1 |
| GSM1192226 |
Bcell_activated_30min, AE2 |
| GSM1192227 |
Bcell_activated-gp120pp_30min, AE3 |
| GSM1192228 |
Bcell_activated-gp120m_30min, AE4 |
| GSM1192229 |
Bcell_activated_3hrs, AE5 |
| GSM1192230 |
Bcell_activated-gp120pp_3hrs, AE6 |
| GSM1192231 |
Bcell_activated-gp120m_3hrs, AE7 |
| GSM1192232 |
Bcell_activated_6hrs, AE8 |
| GSM1192233 |
Bcell_activated-gp120pp_6hrs, AE9 |
| GSM1192234 |
Bcell_activated-gp120m_6hrs, AE10 |
| GSM1192235 |
Bcell_mock_0hrs, AF1 |
| GSM1192236 |
Bcell_activated_30min, AF2 |
| GSM1192237 |
Bcell_activated-gp120pp_30min, AF3 |
| GSM1192238 |
Bcell_activated-gp120m_30min, AF4 |
| GSM1192239 |
Bcell_activated_3hrs, AF5 |
| GSM1192240 |
Bcell_activated-gp120pp_3hrs, AF6 |
| GSM1192241 |
Bcell_activated-gp120m_3hrs, AF7 |
| GSM1192242 |
Bcell_activated_6hrs, AF8 |
| GSM1192243 |
Bcell_activated-gp120pp_6hrs, AF9 |
| GSM1192244 |
Bcell_activated-gp120m_6hrs, AF10 |
| GSM1192245 |
Bcell_mock_6hrs, AG1 |
| GSM1192246 |
Bcell_activated_6hrs, AG2 |
| GSM1192247 |
Bcell_activated-gp120PP_6hrs, AG3 |
| GSM1192248 |
Bcell_activated-gp120m_6hrs, AG4 |
| GSM1192249 |
Bcell_mock_6hrs, AH1 |
| GSM1192250 |
Bcell_activated_6hrs, AH2 |
| GSM1192251 |
Bcell_activated-gp120PP_6hrs, AH3 |
| GSM1192252 |
Bcell_activated-gp120m_6hrs, AH4 |
| GSM1192253 |
Bcell_mock_6hrs, AJ1 |
| GSM1192254 |
Bcell_activated_6hrs, AJ2 |
| GSM1192255 |
Bcell_activated-gp120PP_6hrs, AJ3 |
| GSM1192256 |
Bcell_activated-gp120m_6hrs, AJ4 |
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| Relations |
| BioProject |
PRJNA212697 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE49019_RAW.tar |
176.3 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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