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Sample GSM1192226 Query DataSets for GSM1192226
Status Public on Oct 17, 2013
Title Bcell_activated_30min, AE2
Sample type RNA
 
Source name Bcell, activated, 30min
Organism Homo sapiens
Characteristics disease status: healthy
cell type: B cells
gp120 treatment: none
time point: 30min
Treatment protocol Isolated B cells were treated with gp120 proteins of different affinity or mock protein. The exposure time points included 30min, 3hrs and 6hrs.
Growth protocol Freshly isolated PBMCs were obtained from healthy donors and separated by Ficoll-Hypaque. Purified CD4+ T cells or B cells were obtained by negative selection using magnetic beads (StemCell Technologies, Vancouver, Canada). Cultured CD4+ cells were activated with OKT3, IL2 (20IU/ml) and all-trans retinoic acid (RA, 10nM) unless otherwise specified. RA was obtained from Sigma (St. Louis, MO) and discarded 1 month after reconstitution.
Extracted molecule total RNA
Extraction protocol cDNA of each sample was generated with Ambion WT Expression Kit.
Label biotin
Label protocol cRNA was labelled according to the manufacturer's recommended standard protocol for the Affymetrix Human Gene 1.0 ST microarray.
 
Hybridization protocol Labeled cRNA was hybridized to the Affymetrix Human Gene 1.0 ST microarray according to the manufacturer's recommended standard protocol.
Scan protocol Hybridized Affymetrix human gene microarrays were scanned according to the manufacturer's recommended standard protocol.
Description AE2
Data processing Gene expression profiles were analyzed using Partek Genomic Suite 6.6.
 
Submission date Jul 18, 2013
Last update date Sep 01, 2016
Contact name Richard Lempicki
Organization name NCI-Frederick
Lab Laboratory of Immunopathogenesis and Bioinformatics
Street address 1050 Boyles Street / P.O. Box B
City Frederick
State/province MD
ZIP/Postal code 21702
Country USA
 
Platform ID GPL6244
Series (1)
GSE49019 HIV-1 gp120 impairs B cell proliferation by inducing TGF-β1 production and FcRL4 expression via an α4β7-dependent mechanism
Relations
Reanalyzed by GSE86357

Data table header descriptions
ID_REF
VALUE Normalized expression value in log2 scale

Data table
ID_REF VALUE
8157281 4.59231
7997332 5.7484
8072798 8.65252
7972808 4.91812
8157283 8.37161
7947375 5.38568
7972810 7.93121
8097335 5.38816
8047377 7.05241
7997336 4.98257
8047379 5.18219
8047381 8.87145
8022856 4.82687
8157300 4.88945
8072817 5.42082
7997346 4.84585
8107348 6.82389
8157308 5.52017
8107350 8.11348
8072825 7.5696

Total number of rows: 28869

Table truncated, full table size 447 Kbytes.




Supplementary file Size Download File type/resource
GSM1192226_CC-1-a4b7-B-AE2_2-1-Hugene-1_0.CEL.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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