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Sample GSM1192253 Query DataSets for GSM1192253
Status Public on Oct 17, 2013
Title Bcell_mock_6hrs, AJ1
Sample type RNA
 
Source name Bcell, mock, 6hrs
Organism Homo sapiens
Characteristics disease status: healthy
cell type: B cells
gp120 treatment: mock
time point: 6hrs
Treatment protocol Isolated B cells were treated with gp120 proteins of different affinity or mock protein. The exposure time points included 30min, 3hrs and 6hrs.
Growth protocol Freshly isolated PBMCs were obtained from healthy donors and separated by Ficoll-Hypaque. Purified CD4+ T cells or B cells were obtained by negative selection using magnetic beads (StemCell Technologies, Vancouver, Canada). Cultured CD4+ cells were activated with OKT3, IL2 (20IU/ml) and all-trans retinoic acid (RA, 10nM) unless otherwise specified. RA was obtained from Sigma (St. Louis, MO) and discarded 1 month after reconstitution.
Extracted molecule total RNA
Extraction protocol cDNA of each sample was generated with Ambion WT Expression Kit.
Label biotin
Label protocol cRNA was labelled according to the manufacturer's recommended standard protocol for the Affymetrix Human Gene 1.0 ST microarray.
 
Hybridization protocol Labeled cRNA was hybridized to the Affymetrix Human Gene 1.0 ST microarray according to the manufacturer's recommended standard protocol.
Scan protocol Hybridized Affymetrix human gene microarrays were scanned according to the manufacturer's recommended standard protocol.
Description AJ1
Data processing Gene expression profiles were analyzed using Partek Genomic Suite 6.6.
 
Submission date Jul 18, 2013
Last update date Sep 01, 2016
Contact name Richard Lempicki
Organization name NCI-Frederick
Lab Laboratory of Immunopathogenesis and Bioinformatics
Street address 1050 Boyles Street / P.O. Box B
City Frederick
State/province MD
ZIP/Postal code 21702
Country USA
 
Platform ID GPL6244
Series (1)
GSE49019 HIV-1 gp120 impairs B cell proliferation by inducing TGF-β1 production and FcRL4 expression via an α4β7-dependent mechanism
Relations
Reanalyzed by GSE86357

Data table header descriptions
ID_REF
VALUE Normalized expression value in log2 scale

Data table
ID_REF VALUE
8157281 4.0689
7997332 7.16823
8072798 8.1027
7972808 4.78063
8157283 7.9368
7947375 4.89547
7972810 7.89306
8097335 4.80703
8047377 6.67998
7997336 4.07148
8047379 5.43801
8047381 8.25062
8022856 4.3362
8157300 4.28112
8072817 4.73399
7997346 4.77233
8107348 5.78041
8157308 4.93633
8107350 7.16933
8072825 7.07484

Total number of rows: 28869

Table truncated, full table size 447 Kbytes.




Supplementary file Size Download File type/resource
GSM1192253_CC-1-a4b7_B-AJ1_5-1-HuGene.CEL.gz 4.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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