|
| Status |
Public on Oct 17, 2013 |
| Title |
Bcell_activated-gp120pp_6hrs, AF9 |
| Sample type |
RNA |
| |
|
| Source name |
Bcell, activated, gp120++, 6hrs
|
| Organism |
Homo sapiens |
| Characteristics |
disease status: healthy
cell type: B cells
gp120 treatment: ++
time point: 6hrs
|
| Treatment protocol |
Isolated B cells were treated with gp120 proteins of different affinity or mock protein. The exposure time points included 30min, 3hrs and 6hrs.
|
| Growth protocol |
Freshly isolated PBMCs were obtained from healthy donors and separated by Ficoll-Hypaque. Purified CD4+ T cells or B cells were obtained by negative selection using magnetic beads (StemCell Technologies, Vancouver, Canada). Cultured CD4+ cells were activated with OKT3, IL2 (20IU/ml) and all-trans retinoic acid (RA, 10nM) unless otherwise specified. RA was obtained from Sigma (St. Louis, MO) and discarded 1 month after reconstitution.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
cDNA of each sample was generated with Ambion WT Expression Kit.
|
| Label |
biotin
|
| Label protocol |
cRNA was labelled according to the manufacturer's recommended standard protocol for the Affymetrix Human Gene 1.0 ST microarray.
|
| |
|
| Hybridization protocol |
Labeled cRNA was hybridized to the Affymetrix Human Gene 1.0 ST microarray according to the manufacturer's recommended standard protocol.
|
| Scan protocol |
Hybridized Affymetrix human gene microarrays were scanned according to the manufacturer's recommended standard protocol.
|
| Description |
AF9
|
| Data processing |
Gene expression profiles were analyzed using Partek Genomic Suite 6.6.
|
| |
|
| Submission date |
Jul 18, 2013 |
| Last update date |
Sep 01, 2016 |
| Contact name |
Richard Lempicki |
| Organization name |
NCI-Frederick
|
| Lab |
Laboratory of Immunopathogenesis and Bioinformatics
|
| Street address |
1050 Boyles Street / P.O. Box B
|
| City |
Frederick |
| State/province |
MD |
| ZIP/Postal code |
21702 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE49019 |
HIV-1 gp120 impairs B cell proliferation by inducing TGF-β1 production and FcRL4 expression via an α4β7-dependent mechanism |
|
| Relations |
| Reanalyzed by |
GSE86357 |
