NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1192223 Query DataSets for GSM1192223
Status Public on Oct 17, 2013
Title Bcell_activated-gp120pp_6hrs, AD9
Sample type RNA
 
Source name Bcell, activated, gp120++, 6hrs
Organism Homo sapiens
Characteristics disease status: healthy
cell type: B cells
gp120 treatment: ++
time point: 6hrs
Treatment protocol Isolated B cells were treated with gp120 proteins of different affinity or mock protein. The exposure time points included 30min, 3hrs and 6hrs.
Growth protocol Freshly isolated PBMCs were obtained from healthy donors and separated by Ficoll-Hypaque. Purified CD4+ T cells or B cells were obtained by negative selection using magnetic beads (StemCell Technologies, Vancouver, Canada). Cultured CD4+ cells were activated with OKT3, IL2 (20IU/ml) and all-trans retinoic acid (RA, 10nM) unless otherwise specified. RA was obtained from Sigma (St. Louis, MO) and discarded 1 month after reconstitution.
Extracted molecule total RNA
Extraction protocol cDNA of each sample was generated with Ambion WT Expression Kit.
Label biotin
Label protocol cRNA was labelled according to the manufacturer's recommended standard protocol for the Affymetrix Human Gene 1.0 ST microarray.
 
Hybridization protocol Labeled cRNA was hybridized to the Affymetrix Human Gene 1.0 ST microarray according to the manufacturer's recommended standard protocol.
Scan protocol Hybridized Affymetrix human gene microarrays were scanned according to the manufacturer's recommended standard protocol.
Description AD9
Data processing Gene expression profiles were analyzed using Partek Genomic Suite 6.6.
 
Submission date Jul 18, 2013
Last update date Sep 01, 2016
Contact name Richard Lempicki
Organization name NCI-Frederick
Lab Laboratory of Immunopathogenesis and Bioinformatics
Street address 1050 Boyles Street / P.O. Box B
City Frederick
State/province MD
ZIP/Postal code 21702
Country USA
 
Platform ID GPL6244
Series (1)
GSE49019 HIV-1 gp120 impairs B cell proliferation by inducing TGF-β1 production and FcRL4 expression via an α4β7-dependent mechanism
Relations
Reanalyzed by GSE86357

Data table header descriptions
ID_REF
VALUE Normalized expression value in log2 scale

Data table
ID_REF VALUE
8157281 4.4855
7997332 5.64855
8072798 8.22931
7972808 4.98338
8157283 8.34621
7947375 5.25486
7972810 7.50559
8097335 5.42776
8047377 6.6477
7997336 4.94054
8047379 5.30452
8047381 8.77343
8022856 4.59835
8157300 4.82566
8072817 5.17661
7997346 4.91242
8107348 6.55608
8157308 5.47227
8107350 8.06299
8072825 7.41018

Total number of rows: 28869

Table truncated, full table size 447 Kbytes.




Supplementary file Size Download File type/resource
GSM1192223_CC-1-a4b7-B-AD9_19-1-Hugene-1_0.CEL.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap