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Series GSE22775 Query DataSets for GSE22775
Status Public on Jul 21, 2010
Title Musashi 2 regulates normal hematopoiesis and accelerates leukemogenesis (Leukemia cell lines)
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We demonstrate that Msi2 is the predominant form expressed in hematopoietic stem cells (HSC), and its knockdown leads to reduced engraftment and depletion of HSCs in vivo. Overexpression of Msi2 in a mouse model increases HSC cell cycle progression and cooperates with BCR-ABL1 to induce an aggressive leukemia. MSI2 is over-expressed in human myeloid leukemia, and expression levels directly correlate with decreased patient survival, thereby defining MSI2 expression as a novel prognostic marker in acute myeloid leukemia (AML). Depletion of MSI2 in human myeloid leukemia cells leads to decreased proliferation and apoptosis. These data implicate the MSI2 RNA binding protein in myeloid leukemogenesis and identify a novel potential target for therapy in AML.
Overall design We transduced four human leukemia cell lines with lentiviral shRNA vectors targeting MSI2. Hybridizations of treated and control samples from each cell line were dye swap replicated
Contributor(s) Kharas MG, Lengner CJ, Al-Shahrour F, Bullinger L, Ball B, Zaidi S, Morgan K, Tam W, Paktinat M, Okabe R, Gozo M, Einhorn W, Lane SW, Scholl C, Fröhling S, Fleming M, Ebert BL, Gilliland DG, Jaenisch R, Daley GQ
Citation(s) 20616797, 29221109
Submission date Jul 06, 2010
Last update date Jun 13, 2019
Contact name Fatima Al-Shahrour
Organization name Broad Institute of MIT and Harvard
Department Cancer program
Street address 7 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (8)
GSM563210 Human chronic myeloid leukemic cell line LAMA-84.1
GSM563211 Human chronic myeloid leukemic cell line LAMA-84.2
GSM563212 Human chronic myeloid leukemic cell line ARA-230.1
This SubSeries is part of SuperSeries:
GSE22778 Musashi 2 regulates normal hematopoiesis and accelerates leukemogenesis
BioProject PRJNA129145

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE22775_RAW.tar 132.5 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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