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Sample GSM563214 Query DataSets for GSM563214
Status Public on Jul 21, 2010
Title Human acute myeloid leukaemia cell line NOMO-I.1
Sample type RNA
 
Channel 1
Source name NOMO-I, control
Organism Homo sapiens
Characteristics cell line: NOMO-I
treatment: Control
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions
Label Cy3
Label protocol Quick Amp Agilent protocol
 
Channel 2
Source name NOMO-I, MSI2 shRNA
Organism Homo sapiens
Characteristics treatment: MSI2 shRNA
cell line: NOMO-I
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions
Label Cy5
Label protocol Quick Amp Agilent protocol
 
 
Hybridization protocol Agilent protocol
Scan protocol Agilent protocol
Description Scramble Control shRNA (Cy3) vs msi2 shRNA hairpin1 (Cy5), NOMO-I
Data processing Image intensity data were recorded and transferred into text files using Feature Extraction software (version 8.5). Limma R/BioC package was used for background correction. Loess normalization was applied within arrays and between arrays using a quantile normalization procedure, and log transformed
 
Submission date Jul 06, 2010
Last update date Jul 21, 2010
Contact name Fatima Al-Shahrour
E-mail(s) shahrour@broadinstitute.org
Organization name Broad Institute of MIT and Harvard
Department Cancer program
Street address 7 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
 
Platform ID GPL4133
Series (2)
GSE22775 Musashi 2 regulates normal hematopoiesis and accelerates leukemogenesis (Leukemia cell lines)
GSE22778 Musashi 2 regulates normal hematopoiesis and accelerates leukemogenesis

Data table header descriptions
ID_REF
VALUE lowess normalized

Data table
ID_REF VALUE
1 0.246493369
2 -0.409452621
3 -0.52675996
4 0.470068984
5 0.067108971
6 -0.104054755
7 -0.519605845
8 0.124574474
9 0.465722317
10 0.464890067
11 -0.30366024
12 0.081952969
13 -0.07161839
14 -0.088051575
15 0.02429846
16 0.346383576
17 0.190219787
18 0.094277781
19 0.217576604
20 0.254133012

Total number of rows: 45015

Table truncated, full table size 793 Kbytes.




Supplementary file Size Download File type/resource
GSM563214_251485042609_200907021136_S01_GE2-v5_10_Apr08_1_4.txt.gz 15.7 Mb (ftp)(http) TXT
Processed data included within Sample table

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